Physical stimulation of mammalian cells using micro-bead impact within a microfluidic environment to enhance growth rate |
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Authors: | Tae-Jin Kim Su-Jin Kim Hyo-Il Jung |
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Affiliation: | (1) School of Mechanical Engineering, Yonsei University, Seoul, South Korea;(2) National Core Research Center for Nanomedical Technology, Yonsei University, Seoul, South Korea |
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Abstract: | Various cell stimulation experiments have been traditionally conducted regarding observation of cells to specific stimulative
factors, and in light of this area of study, we report a new method by utilizing micro-beads. HeLa cells and MC3T3 cells are
cultured in straight PDMS (polydimethylsiloxane) microfluidic devices and are stimulated by sterilized polystyrene micro-beads.
Cell culture medium either with or without micro-beads are introduced in microfluidic cell culturing chambers at a specific
time interval, and stimulated cells are observed using an inverted microscope. The results show that cells exposed under micro-bead
stimulation perform at a higher growth rate than those under normal conditions. This paper demonstrates that micro-beads can
be used as a physical stimulation factor and affect cell growth behaviors. |
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Keywords: | HeLa cell MC3T3 cell Micro-bead stimulation Microfluidic channel |
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