| c-FLIP mRNA在大肠癌细胞中的表达及其与Fas抗原表达的相关性 |
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| 引用本文： | 高文超,王元和,李莉,王强,张玲珍,曹鲁宁,丁尔迅.c-FLIP mRNA在大肠癌细胞中的表达及其与Fas抗原表达的相关性[J].第二军医大学学报,2002,23(1):35-37. |
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| 作者姓名： | 高文超 王元和 李莉 王强 张玲珍 曹鲁宁 丁尔迅 |
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| 作者单位： | 1. 第二军医大学长征医院普通外科,上海,200003
2. 长征医院实验诊断科 |
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| 基金项目： | 国家自然科学基金，39900143， |
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| 摘 要： | 目的:探讨cFLIP(细胞型Fas相关死亡区域蛋白样白介素-1β转换酶抑制蛋白) mRNA在大肠癌细胞中的表达及其与Fas抗原(CD95)表达的相关性.方法:应用RT-PCR方法检测3株人大肠癌细胞株中c-FLIP mRNA的表达,采用间接免疫荧光标记流式细胞术检测大肠癌细胞Fas抗原表达.结果:SW1116及SW620细胞株 c-FLIP mRNA 表达阳性并相对高水平表达Fas抗原,而LoVo细胞株c-FLIP mRNA表达阴性并低水平表达Fas抗原.结论:在大肠癌细胞中,c-FLIP mRNA的表达趋势与Fas抗原一致,提示c-FLIP可能在抑制Fas抗原诱导的细胞凋亡中发挥重要作用.
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| 关 键 词： | CD95、c-FLIP、I-FLICE、大肠癌、流式细胞术 |
| 文章编号： | 0258-879X(2002)01-0035-03 |
| 修稿时间： | 2001年8月6日 |
Expression of c-FLIP mRNA in colorectal cancer cells and its relationship with Fas antigen (CD95) expression |
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| GAO Wen-Chao ,WANG Yuan-He ,LI Li ,WANG Qiang ,ZHANG Ling-Zhen ,CAO Lu-Ning ,DING Er-Xun.Expression of c-FLIP mRNA in colorectal cancer cells and its relationship with Fas antigen (CD95) expression[J].Academic Journal of Second Military Medical University,2002,23(1):35-37. |
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| Authors: | GAO Wen-Chao WANG Yuan-He LI Li WANG Qiang ZHANG Ling-Zhen CAO Lu-Ning DING Er-Xun |
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| Affiliation: | GAO Wen-Chao 1,WANG Yuan-He 1,LI Li 2,WANG Qiang 1,ZHANG Ling-Zhen 2,CAO Lu-Ning 2,DING Er-Xun 1 |
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| Abstract: | Objective:To investigate the expression of c-FLIP mRNA in colorectal cancer cells and its relationship with Fas antigen(CD95)expression.Methods:RT-PCR was employed to detect the expression of c-FLIP mRNA and indirect immunofluoresence label flow cytometry was utilized to assay the Fas antigen expression in 3 colorectal cancer cell lines.Results: c-FLIP mRNA was positive in SW1116 and SW620 cell lines and negative in LoVo cell lines.The expression of Fas antigen was much higher in SW1116 and SW620 cell lines than that in LoVo cell lines.Conclusion:The expression of c-FLIP mRNA had positive correlation with the expression of Fas antigen in colorectal cancer cells,and c-FLIP may significantly inhibit the apoptosis induced by Fas in colorectal cancer cells. |
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| Keywords: | CD95 c-FLIP I-FLICE colorectal neoplasms flow cytometry |
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