尼古丁对人脐静脉内皮细胞凋亡及坏死的影响

目的不同浓度尼古丁对人脐静脉内皮细胞(HUVECs)凋亡及坏死的影响。方法用CD34以免疫组织化学法鉴定HUVECs。通过3种不同浓度的尼古丁(3、30、300 ng/ml)刺激HUVECs 24 h后,用流式细胞仪检测其凋亡及坏死率。结果低浓度尼古丁促进细胞凋亡最强,而随着尼古丁浓度的增加,细胞凋亡率反而逐渐降低,各组差异具有统计学意义(P<0.05);此外,随着尼古丁浓度增加,细胞坏死率也日益增多,各组差异具有统计学意义(P<0.05)。细胞坏死率与尼古丁浓度呈正相关(r=0.675)。结论尼古丁对HUVECs凋亡的影响具有浓度依赖性,细胞坏死率与尼古丁浓度呈正相关。

Effect of nicotine on human umbilical vein endothelial cells

Objective To investigate the effect of nicotine on the mode of human umbilical vein endothelial cells(HUVECs) death.Methods CD34 were used as a marker to identify HUVECs by immunocytochemistry.HUVECs were treated with three different concentrations of nicotine(3,30 and 300 ng/ml) for 24 hours.The rate of apoptosis or necrosis was analyzed with Flow cytometry.Results A low concentration of nicotine(3 ng/ml) was found to promote cell apoptosis.The percentage of apoptosis was significantly decreased when the concentration of nicotine was increased(P<0.05).The reduction of apoptosis was accompanied with an increased in necrosis.The increase in necrosis was positively correlated with an increasing concentration of nicotine(r=0.675).Conclusion Nicotine dose-dependently increases necrosis of human umbilical vein endothelial cells.