玉麦须醇提物对L929细胞氧化应激损伤的保护作用

目的:观察不同剂量玉麦须醇提物(MSE)对照射所致L929细胞氧化应激损伤的保护作用及其可能的机制。方法:⁶⁰Co照射L929细胞后,即分别给予0、10、50、250μg/mL MSE处理,阴性对照组屏蔽照射,检测细胞氧化损伤、抗氧化系统、炎症因子等相关氧化应激指标的变化。结果:⁶⁰Co照射能引起显著的细胞氧化应激损伤,MSE处理后能降低硫代巴比妥酸反应产物(TBARS)含量,提高过氧化氢酶和过氧化物歧化酶的活性、增加GSH/GSSG比值,且存在一定剂量效应关系。同时,MSE能上调Nrf2和γ谷氨酰半胱氨酸合成酶的表达,抑制转化生长因子β(TGF-β)的基因和蛋白表达,以中剂量组(50μg/mL)作用最为显著。结论:一定剂量的MSE可改善由照射引发的细胞氧化应激损伤,Nrf2和TGF-β可能为玉麦须有效的作用靶点。

Protective effects of maize silks ethanol extract on oxidative injury in L929 cells

OBJECTIVE: To investigate the protective effects of maize silks ethanol extract(MSE) against radiation-induced oxidative damage in cells. METHODS: γ-radiation was employed to induce oxidative stress in L929 cells. After radiation,cells were treated immediately with different doses of MSE(10,50,250μg/mL). The variation of oxidative damage indicator,antioxidant system and inflammatory factors were examined. RESULTS: Radiation caused obvious oxidative injury in cells. MSE treatment significantly abolished elevation of thiobarbituric acid reactive substances(TBARS) levels,increased hepatic GSH/GSSG ratio and activities of catalase and superoxide dismutase. Moreover,MSE up-regulated the gene expressions of NF-E2-related factor 2 and gamma glutamyl cysteine synthetase. The gene and protein expressions of transforming growth factor beta(TGF-β)were also inhibited. Interestingly,50μg/mL MSE treatment performed best in our most results. CONCLUSION: These findings proved the protective role of MSE against radiationinduced oxidative injury. Nrf2 and TGF-β could be the potential targets of maize silks.