c-metprotoncogene在结直肠癌肝转移诊断中的应用

背景与目的：早期诊断肝转移对结直肠癌预后有重要作用。本研究探讨c—metprotoncogene（c—met）在结直肠癌组织中的表达，及其与结直肠癌肝转移的关系，评价其在结直肠癌肝转移早期诊断中的应用价值。方法：用免疫组化法检测无肝转移、术时合并肝转移和术后发生肝转移各30例结直肠癌组织、30例癌旁正常粘膜组织中的c—met表达水平，分析与性别、年龄、Dukes分期等临床特征之间的联系，比较无肝转移、术后肝转移、术时肝转移的结直肠癌组织和癌旁正常组织中c—met表达水平的差异。结果：c—met在结直肠癌组织中的表达与性别、年龄、术前CEA和CA19-9无关（P〉0．05），与Dukes分期、淋巴结转移有关（P〈0．05）；c—met在结直肠癌组织中的表达显著高于正常粘膜组织（P〈0．05）；c—met在术时肝转移组中的表达显著高于无肝转移组（P〈0．05），术后肝转移组与无肝转移组中表达差异无显著性（P〉0．05）。结论：c—met过度表达与结直肠癌发生、发展、肝转移相关。

The role of c-met gene in the diagnosis of liver metastasis of colorectal carcinoma

Background and purpose:Early diagnosis of liver metastasis is important to the prognosis of colorectal carcinoma.We investigated the expression of c-metprotoncogene(c-met) in colorectal carcinoma,and the correlation with liver metastasis.Methods:The expression of c-met detected by immunohistochemical method in 90 colorectal carcinoma tissues that included the patients without liver metastasis(n=30),with preoperative liver metastasis(n=30) and with post-operative liver metastasis(n=30).30 surrounding normal colorectal tissues also had been evaluated.The multiple parameters like gender,age,Dukes stages and liver metastasis had also been analyzed to clarify the relationship between them and the expression of c-met.Results:The expression level of c-met in colorectal carcinoma was significantly higher than that in surrounding normal colorectal tissue(P<0.05).The expression level of c-met in colorectal carcinoma without liver metastasis was significantly lower than that with preoperative liver metastasis(P<0.05).There was no significant difference between the group without liver metastasis and the group with post-operative liver metastasis(P>0.05).The expression level of c-met had no correlation with gender,age or the preoperative serum CEA and CA19-9 levels in colorectal carcinoma(P>0.05).The expression level of c-met had correlation with Dukes stages and lymph node metastasis.Conclusions:c-met might play a role in predicting the prognosis in colorectal carcinoma and further study is needed to clarify the role of cmet in the process of metastasis of colorectal carcinoma.