| 血液保存对红细胞携氧功能及能量代谢的影响 |
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| 引用本文： | 李遥金,王翔,兰珂.血液保存对红细胞携氧功能及能量代谢的影响[J].中国输血杂志,2009,22(6). |
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| 作者姓名： | 李遥金 王翔 兰珂 |
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| 作者单位： | 重庆大学生物工程学院,重庆,400030 |
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| 基金项目： | 国家自然科学基金项目(编号:10572159);;重庆市科委科技计划项目攻关项目(编号:2006ba5010) |
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| 摘 要： | 目的探讨血液保存时间和过滤、离心等前处理操作对红细胞携氧功能及能量代谢的影响。方法取不同保存期血样用血氧分析仪检测携氧能力并同时测定葡萄糖、Na+、K+及乳酸脱氢酶等能量代谢相关指标。结果全血和悬浮红细胞的有效携氧量随保存时间延长逐渐减少,在第1周分别下降了27.5%和19.2%。到保存末期,全血的有效携氧量仅为"新鲜血"的39%,悬浮红细胞也只有采血当天的51%。P50值在保存期总体呈减小趋势,在开始2周减少较快,随后变化不明显。葡萄糖逐渐消耗;乳酸脱氢酶随时间逐渐增加,全血到保存末期为采血时的近3倍。Na+浓度呈进行性下降,K+浓度逐渐增加,到保存末期悬浮红细胞K+增加了3倍。结论血液保存时间和前处理操作等对血红细胞携氧能力及能量代谢有重要影响,过滤、离心等操作会对红细胞造成一次性损伤。
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| 关 键 词： | 血液保存 红细胞 携氧功能 |
Effect of blood storage on RBC oxygen-carrying function and energy metabolism |
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| LI Yaojin,WANG Xiang,LAN Ke.Effect of blood storage on RBC oxygen-carrying function and energy metabolism[J].Chinese Journal of Blood Transfusion,2009,22(6). |
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| Authors: | LI Yaojin WANG Xiang LAN Ke |
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| Affiliation: | LI Yaojin,WANG Xiang,LAN Ke. Bioengineering College of Chongqing University,Chongqing 400030,China |
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| Abstract: | Objective To evaluate the impact of storage time and pre-treatment process on the oxygen-carrying function and energy metabolism of red blood cells.Methods Oxygen-carrying capacity was measured by HEMOX-ANALYZER,and the concentrations of glucose,Na+,K+ and lactate dehydrogenase were also detected at different storage time. Results The effective oxygen-carrying volume of whole blood and suspended RBC decreased by 27.5% and 19.2%,respectively after 1 week,and at the end of preservation,the volume decreased to... |
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| Keywords: | Blood storage Red blood cell Oxygen-carrying function |
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