醋酸氯己定和三氯羟苯醚对义齿软衬材料微生物黏附抑制的研究

目的 探讨醋酸氯己定和三氯羟苯醚两种消毒液对减少老年人常用的义齿软衬材料表面微生物黏附的效果,以预防义齿性口腔炎的发生.方法 对树脂类软衬材料和硅橡胶类软衬材料经两种不同的消毒液(0.2%醋酸氯己定溶液和0.1%三氯羟苯醚溶液)浸泡5 min后,观察材料表面黏附的3种微生物(变形链球菌、黏性放线菌和白色念珠菌)菌落计数的变化.结果 树脂类软衬材料本身白色念珠菌黏附菌落数少于硅橡胶类软衬材料,分别为(121.0±7.0)× 105cfu/ml和(208.8±8.6)×105cfu/ml,差异有统计学意义(P<0.05),而变形链球菌和黏性放线菌在二者表面黏附情况的差异无统计学意义.经醋酸氯己定、三氯羟苯醚浸泡后树脂类和硅橡胶类软衬材料表面变形链球菌菌落计数减少,分别为(87.1±4.3)×105cfu/ml、(61.6±7.9)×105cfu/ml和(42.1±8.2)×105cfu/ml、(21.3±4.3)×105cfu/ml;白色念珠菌落计数减少,分别为(11.6±3.6)×105cfu/ml、(11.1±3.7)×105cfu/ml和(41.6±3.0)×105cfu/ml、(44.6±4.1)×105cfu/ml,差异均有统计学意义(P<0.01).而醋酸氯己定和三氯羟苯醚对黏性放线菌菌落计数无影响,两种消毒液作用效果差异无统计学意义(P>0.05).结论 用醋酸氯己定和三氯羟苯醚浸泡树脂类软衬材料和硅橡胶类软衬材料均可以有效预防软衬材料表面变形链球菌和白色念珠菌黏附.

Preliminary research of chlorhexidine acetate and triclosan on inhibition of microorganism adhesion on soft-lining materials

Objective To explore the effects of chlorhexidine aeetate and trielosan on inhibition of microorganism adhesion on soft denture-lining materials. Methods Silicone rubber soft denture- lining material and resin soft denture-lining material were soaked in 0. 2% chlorhexidine acetate and 0. 1% trielosan for 5 minutes. Then the colony numbers of three different microorganisms (streptococcus mutans, actinomyces viscosus, candida albicans) adhering to soft denture-lining materials were counted. Results The colony numbers of candida albicans were (121.0±7. 0) × 105 cfu/ml in resin soft denture-lining material and (208. 8±8. 6) × 105cfu/ml in silicone rubber soft denture-lining material (P<0. 05). But there were no differences in colony numbers of streptococcus mutans and actinomyces viseosus. After soaked in chlorhexidine acetate and triclosan, the colony numbers of streptococcus mutans were significantly reduced to (87.1±4. 3)× 105cfu/ml, (61.6± 7.9) × 105cfu/ml, (42.1±8.2) × 105cfu/ml and (21.3±4.3)× 105cfu/ml, and the colony numbers of candida albicans were significantly reduced to (11.6±3.6) × 105cfu/ml, (11.1±3. 7) × 105cfu/ml, (41.6±3.0) × 105cfu/ml and (44. 6±4.1)× 105cfu/ml(all P<0. 01). However, chlorhexidine acetate and triclosan had no effects on actinomyces viscosus. There were no significant differences in the action effects between the two detergents (P>0. 05). Conclusions Chlorhexidine acetate and trielosan can effectively inhibit the adhesion of microorganism on denture-lining materials, which are useful in clinic.