| 抗人卵巢癌单链抗体噬菌体表面呈现文库的构建、初步筛选及鉴定 |
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| 引用本文： | 杜辉,王建,辛晓燕,郑维国.抗人卵巢癌单链抗体噬菌体表面呈现文库的构建、初步筛选及鉴定[J].第四军医大学学报,2003,24(6):536-539. |
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| 作者姓名： | 杜辉 王建 辛晓燕 郑维国 |
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| 作者单位： | 第四军医大学西京医院妇产科,陕西,西安,710033 |
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| 摘 要： | 目的：构建抗人卵巢癌全套单链抗体基因噬菌体表面呈现库。方法：利用噬菌体表面呈现技术，构建基因库。经过panning筛选富集后，用ELISA方法检验抗原结合活性。结果：从单个噬菌体克隆中筛选到26个具有抗原结合活性的阳性克隆。结论：噬菌体表面呈现技术为构建抗人卵巢癌全套单链抗体基因噬菌体表面呈现库提供了一条更为有效的新途径。
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| 关 键 词： | 噬菌体表面呈现 单链抗体 卵巢肿瘤 |
| 文章编号： | 1000-2790(2003)06-0536-04 |
| 修稿时间： | 2002年9月2日 |
Construction and preliminary selection and identification of phage display library of anti-human ovarian cancer single chain Fv antibody |
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| Abstract: | AIM: To construct the phage display library of anti human ovarian cancer single chain Fv antibody. METHODS: The ScFv gene library was constructed by phage display technology. The affinity to antigen was detected by ELISA after panning. RESULTS: Twenty six antigen binding clones were identified from 94 individual phagemid clones. CONCLUSION: In the production of engineering antibodies against human cancer, this strategy may provide an alternative approach. |
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| Keywords: | phage display single chain antibodies ovarian neoplasms |
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