| 一株蛋白酶产生菌的分离鉴定 |
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| 引用本文： | 李超敏,石晓,窦会娟,赵永敢.一株蛋白酶产生菌的分离鉴定[J].食品工程,2008(3):48-50. |
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| 作者姓名： | 李超敏 石晓 窦会娟 赵永敢 |
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| 作者单位： | 漯河医学高等专科学校食品工程系,漯河,462002 |
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| 摘 要： | 从郑州奶牛场土壤样品中分离到一株芽孢杆菌，命名为A—19菌株。该菌株有较高的蛋白酶活性，37℃、pH7．0培养48h后，其蛋白酶活力可达63．5U/mL。菌体生长专性需氧，有运动性，粗糙型菌落，氧化酶试验阳性，DNA（G＋C）的摩尔分数为47％。该菌株的16SrDNA序列与芽孢杆菌属有很高的同源性，Bacillus vallismortis（99．61％），B．subtilis（98．81％），B．amyloliquefaciens（98．95％），B．licheniformis（97．93％）。通过其生理生化特征和16S rDNA序列相似性的系统发育分析，确定A—19菌株属于芽孢杆菌属。该菌的16S rDNA序列已被GenBank收录，序列登陆号为EU561347。
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| 关 键 词： | 蛋白酶 芽匏杆菌 16S RDNA 系统发育 |
Screening and identification of microorganism producing protease |
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| LI Chao-min,SHI Xiao,DOU Hui-juan,ZHAO Yong-gan.Screening and identification of microorganism producing protease[J].Food Engineering,2008(3):48-50. |
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| Authors: | LI Chao-min SHI Xiao DOU Hui-juan ZHAO Yong-gan |
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| Affiliation: | LI Chao-min, SHI Xiao, DOU Hui-juan ,ZHAO Yong-gan (Food Engineering Department,Luohe Medical College,Luohe 462002,China) |
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| Abstract: | An endospore-forming bacterium, designated strain A-19,was isolated from a milk cow field sample in Zhengzhou. It presents remarkable protease activity. The strain was strictly aerobic, motile, spore forming and rod shaped,oxidase-positive. The DNA （G＋C） content was 47 %. Strain A-19 exhibited high 16S rDNA similarity with its closest neighbors.Bacillus vallismortis （99.61% ）, B. subtilis （98.81% ）, B.amyloliquefaciens （98.95 %）, B. licheniformis （97.93 %）. The phenotypic and genotypic characteristics indicate that strain A-19 belongs to the genus Bacillus. Its protease activity was 63.5 U / mL at 37 ℃、pH7.0. The GenBank accession number for the sequence reported in this paper is EU561347. |
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| Keywords: | 16S rDNA |
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