| 活血健脾补肾三法对骨髓间充质干细胞移植在结肠炎大鼠肠道定位的影响 |
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| 引用本文： | 张永锋,杨晋,吴正治,张莉,胡银清,杨敏.活血健脾补肾三法对骨髓间充质干细胞移植在结肠炎大鼠肠道定位的影响[J].中国中西医结合消化杂志,2010,18(1). |
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| 作者姓名： | 张永锋 杨晋 吴正治 张莉 胡银清 杨敏 |
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| 作者单位： | 1. 深圳市第二人民医院中西医结合临床研究所,广东,深圳,518035
2. 湖北中医学院,湖北,武汉,430061 |
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| 基金项目： | 深圳市科技计划资助项目(200802028) |
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| 摘 要： | 目的]探讨中医活血、健脾、补肾三法对骨髓间充质干细胞(MSC)移植在结肠炎大鼠肠道定位的影响。方法]雌性SD大鼠以5%2、4、6-三硝基苯磺酸(TNBs)诱导形成结肠炎模型,造模后随机分为模型对照组、MSC移植组、活血组、健脾组、补肾组,第2天将体外分离培养的雄性SD大鼠MSC经尾静脉注射到各组雌性大鼠体内。疗程结束后取各组大鼠结肠组织,以Y染色体的性别决定区段sry作为标志,以确定MSC的肠道定植情况。结果]经PCR扩增后,凝胶电泳可以检测到结肠组织雄性性别特异性基因区段sry,MSC移植组阳性率为20%,活血组为35%,健脾组为25%,补肾组为25%。结论]活血法代表方药桃红四物汤较明显促进MSC在结肠炎大鼠肠道定位。
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| 关 键 词： | 结肠炎 溃疡性 骨髓间充质干细胞 移植 中医药疗法 |
Effect of promoting blood flow, invigorating spleen or kidney recipe on localization of MSC in the Colon of Ulcerative Colitis after transplantation |
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| ZHANG Yong-feng,YANG Jin,WU Zheng-zhi,ZHANG Li,HU Yin-qing,YANG Min.Effect of promoting blood flow, invigorating spleen or kidney recipe on localization of MSC in the Colon of Ulcerative Colitis after transplantation[J].Chinese Journal of Integrated Traditional and Western Medicine on Digestion,2010,18(1). |
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| Authors: | ZHANG Yong-feng YANG Jin WU Zheng-zhi ZHANG Li HU Yin-qing YANG Min |
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| Affiliation: | ZHANG Yong-feng1,YANG Jin2,WU Zheng-zhi1,ZHANG Li1,HU Yin-qing1,YANG Min1(1Shenzhen Clinical Institute of Integrated TCM , Western Medicine,Shenzhen Second Peoples Hospital,Shenzhen 518035,China,2Hubei College ofTraditional Chinese Medicine,Wuhan 430061,China) |
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| Abstract: | Objective]To explore the effect of promoting blood flow,invigorating spleen or kidney recipe on localization of bone marrow mesenchymal stem cell(MSC)in the colon of ulcerative colitis(UC)after transplantation.Methods]SD female rats were randomly divided into the model group,the MSC transplantation group,promoting blood fiow group,invigorating the spleen group and invigorating the kidney group after the colitis model were induced by Trinitrobenzene sulphonic acid(TNBs).The MSC from SD male rats were purif... |
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| Keywords: | ulcerative colitis bone marrow mesenchymal stem cell transplantation traditional chinese medicine therapy |
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