胃癌组织P53、P63蛋白表达及细胞凋亡的研究

目的：探讨P53、P63蛋白表达及细胞凋亡在胃癌发生发展中的可能机制。方法：选择57例手术切除的胃癌及胃癌旁组织,用免疫组化方法检测组织中的P53、P63蛋白表达,同时用Tunel方法检测胃癌组织中的凋亡细胞。结果：胃癌组织P53、P63蛋白表达率显著高于胃癌旁组织（P〈0.05）;低、未分化型腺癌表达率显著高于高、中分化型腺癌（P〈0.05）;有淋巴转移显著高于无转移组（P〈0.05）;P53、P63高表达的癌组织,细胞凋亡指数减少,早期胃癌低于晚期胃癌（P〈0.05）。结论：胃癌组织P53、P63蛋白高表达,可能延缓细胞凋亡;细胞凋亡机制障碍可能是胃癌增生失控的基础。

Study on the expression of P53,P63 protein and cellular apoptosis in gastric carcinoma

Objective: To study the expression of P53,P63 protein expression and cellular apoptosis in gastric carcinoma.Methods: 57 cases of gastric carcinoma and 20 normal controls were recruited in this study.The tumor tissues and para-cancer tissues resected surgically were examinated.The expression of P53 and P63 protein were detected immunohistochemically,and the apoptosis was shown by tunnel labeling method.Results: Expressions of P53 and P63 in the gastric cancer tissue were significantly higher than those in the paracancer tissue(P<0.05),low differentiated adenocarcinomas showed higher expression rate than those of moderate and high differentiated(P<0.05),and cases with lymph node metastasis had higher expression of P53 P63 than those with no lymph node metastasis(P<0.05).Moreover,the AI(apotopsis index) decreased in the cancer tissues with high expression levels of p53 and P63.The AI of early stage gastric cancer were lower than end stage(P<0.05).Conclusions: The higher expressions of P53 and P63 in the gastric carcinoma may be contributed to the inhibition of cell apoptosis,and the abnormal mechanism of apoptosis may lead to the proliferation of gastric cancer being out of control.