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藏灵菇乳中具有优良抗氧化活性乳酸菌的筛选鉴定
引用本文:罗章,陈历水,陈历俊,刘继超,姜铁民.藏灵菇乳中具有优良抗氧化活性乳酸菌的筛选鉴定[J].食品科学,2015,36(3):109-113.
作者姓名:罗章  陈历水  陈历俊  刘继超  姜铁民
作者单位:1.西藏大学农牧学院食品科学学院,西藏 林芝 860000;2.中粮营养健康研究院,品牌食品研发中心,北京 102209;; 3.北京三元食品股份有限公司科研中心,北京 100086
基金项目:国家高技术研究发展计划(863计划)项目(2011AA100903)
摘    要:为了得到具有抗氧化活性的乳酸菌,采用1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)法和抑制脂质过氧化法对11 株来源于西藏藏灵菇乳的乳酸菌抗氧化活性进行测定,包括它们的完整细胞和无细胞提取物,发现HN05菌株有较好的抗氧化活性。根据菌株的表型、生理生化特征和基因型的特性,初步将HN05菌株鉴定为清酒乳杆菌清酒亚种(Lactobacillus sakei subsp. sakei)。

关 键 词:藏灵菇乳  抗氧化活性  乳酸菌  鉴定  

Screening and Identification of Lactic Acid Bacteria with High Antioxidant Activity from Tibetan Kefir
LUO Zhang;CHEN Lishui;CHEN Lijun;LIU Jichao;JIANG Tiemin.Screening and Identification of Lactic Acid Bacteria with High Antioxidant Activity from Tibetan Kefir[J].Food Science,2015,36(3):109-113.
Authors:LUO Zhang;CHEN Lishui;CHEN Lijun;LIU Jichao;JIANG Tiemin
Affiliation:1. College of Food Science, Agricultural and Animal Husbandry College of Tibet University, Linzhi 860000, China; 2. Brand Food Center of R&D, COFCO Nutrition and Health Research Institute, Beijing 102209, China; 3. R&D Center, Beijing Sanyuan Food Co. Ltd., Beijing 100086, China
Abstract:This study aimed to obtain lactic acid bacteria (LAB) with high antioxidant activity. Intact cells and cell-free
extracts of 11 LAB isolates from Tibetan Kefir were evaluated for antioxidant activities by 1,1-diphenyl-2-picrylhydrazl
(DPPH) free radical scavenging and linoleic acid peroxidation inhibition assays. Strain HN05 possessing excellent
antioxidant activity was screened. Based on its morphological, biochemical, physiological and genotypic characteristics, this
strain was identified as Lactobacillus sakei subsp. sakei.
Keywords:Tibetan Kefir  antioxidant activity  lactic acid bacteria  identification  
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