| 石家庄地区汉族人群DIS80(pMCT118)位点VNTR基因频率分布调查 |
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| 引用本文： | 丛斌,谷振勇,彭郁葱,姚玉霞.石家庄地区汉族人群DIS80(pMCT118)位点VNTR基因频率分布调查[J].河北医科大学学报,1997(3). |
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| 作者姓名： | 丛斌 谷振勇 彭郁葱 姚玉霞 |
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| 摘 要： | 本文采用ＰＣＲ技术对石家庄地区２１６名无关个体ＤＩＳ８０（ｐＭＣＴ１１８）位点扩增片段长度多态性的等位基因频率进行调查。ＰＣＲ扩增产物采用聚丙烯酰胺凝胶电泳分型及溴化乙锭染色，共检出７０种基因型，２５个等位基因，其中以１８、２４、３０三个等位基因频率最高。将本文结果与日本人群在ＤＩＳ８０（ｐＭＣＴ１１８）位点多态性结果进行比较，石家庄地区汉族人群ＤＩＳ８０（ｐＭＣＴ１１８）位点基因频率分布符合ＨａｒｄｙＷｅｉｎｂｅｒｇ平衡定律，该位点非父排除率为０．７１，个体识别率为０．９，杂合度为０．８５。
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| 关 键 词： | 汉族人群 pMCT118 VNTR PCR |
GENETIC POLYMORPHISMS OF DIS80(pMCT118)IN CHINESE HAN POPULATION OF SHIJIAZHUANG AREA |
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| Cong Bin,Gu Zhenyong,Peng Yucong,Yao Yuxia.GENETIC POLYMORPHISMS OF DIS80(pMCT118)IN CHINESE HAN POPULATION OF SHIJIAZHUANG AREA[J].Journal of Hebei Medical University,1997(3). |
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| Authors: | Cong Bin Gu Zhenyong Peng Yucong Yao Yuxia |
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| Affiliation: | Cong Bin Gu Zhenyong Peng Yucong Yao Yuxia Department of Forensic Medicine |
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| Abstract: | Chinese Han population was studied for the D1S80 (pMCT118)locus .Blood stain samples of 216 unrelated were examined through chelex extraction and the polymerase chain reaction(PCR).After amplification,the genotypes were separated by polyacrylamide gel(PAG) electrophoresis and stained with ethidium bromide.A total of 70 genotypes and 25 alleles were distinguished.Alleles 18,24and 30 were the most common alleles in Chinese Han population.The distribution of the observed genotypes conformed to the Hardy Weinberg equilibrium for the DIS80(pMCT118) system.The chance of exclusion ,discriminating power(DP) and heterozygosity for this system in the Chinese Han population were 0.71 , 0.90 and 0.85 respectively. |
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| Keywords: | Han population pMCT118 VNTR PCR |
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