Pin1基因多态性与喉鳞状细胞癌的相关性

目的探讨肽基脯氨酰顺反异构酶(Pin1)启动子区域的单核苷酸多态性(SNP)位点rs2233678、rs2233679与喉鳞状细胞癌(LSCC)的相关性。方法采用聚合酶链式反应-限制性内切酶片段长度多态性(PCR-RFLP)方法检测95例LSCC患者(病例组)及100例喉良性病变患者(对照组)外周血Pin1基因相关的SNP位点的差异;比较突变位点的基因型频率及等位基因型频率与患者颈淋巴结转移、临床分期的关系。结果 rs2233678多态性位点的基因型频率在病例组外周血中表达高于对照组(P<0.05),其基因型频率分布与淋巴结转移、TNM临床分期分组无关。rs2233679多态性位点的基因型频率分布在病例组和对照组中差异无统计学意义(P>0.05)。结论 rs2233678多态性位点与LSCC易感性有关。rs2233679多态性位点与LSCC易感性无关。

Association between polymorphisms in prolyl isomerase Pin1 and the risk for laryngeal squamous cell carcinoma

Objective To investigate the relationship between the single nucleotide polymorphism(SNP) of Pin1 and the susceptibility of laryngeal squamous cell carcinoma(LSCC). Methods The diversity of Pin1 polymorphisms in peripheral blood of 95 LSCC patients and 100 patients with benign laryngeal diseases was detected by PCR-RFLP.Two selected Pin1 polymorphisms were genotyped.The genotype frequencies and allele type frequencies were compared,and the relationship between their genotype frequencies and clinical characteristics(cervical lymph node metastasis and clinical status) were compared as well. Results The genotype frequencies and allele type frequencies of rs2233678 were significantly different in LSCC patients(P<0.05),which was not related to cervical lymph node metastasis and clinical stages(P>0.05).The distribution of genotype frequencies of rs2233679 was not significant between two groups(P>0.05). ConclusionThe rs2233678 variant genotypes are associated with susceptibility of LSCC.No association exists between the variants rs2233679 and the risk for LSCC.