外源性Rb基因对卵巢癌细胞株生长的抑制作用

目的 探讨外源性Ｒｂ基因对卵巢细胞生长的抑制作用，为卵巢癌的基因治疗提供实验依据，方法 通过腺病毒介导的Ｒｂ基因，于体外转染卵巢癌细胞株ＣＡＯＶ３。应用免疫组织化学，ＤＮＡ琼脂糖电泳等方法，检测外源性Ｒｂ基因的导入及其表达。通过四甲基偶氮唑蓝比色法及流式细胞术等，观察转染Ｒｂ基因的ＣＡＯＶ３细胞的生长情况。结果 重组腺病毒载体有可效于体外转染卵巢癌细胞株ＣＡＯＶ３；转染外源性Ｒｂ基因后，ＣＡＯＶ３

Inhibition of Recombinant Rb Gene Adenovirus Vector on the Growth of Ovarian Cancer Cell Line

OBJECTIVE: To test and study the effect of inhibition on the growth of ovarian cancer cell line CAOV3 transfected by the recombinant Rb gene adenovirus vector. METHODS: The recombinant Rb gene and the LacZ gene in adenovirus vector were constructed and transfected into the CAOV3 cell line separately. The Rb gene expression was detected by immunohistochemical assay and agarose gel electrophoresis, etc. The inhibition on the growth of CAOV3 was tested by flow cytometry and methyl thiazolyl tetrazolium assay, etc. RESULTS: The constructed recombinant Rb gene adenovirus vector could infect cells with high level expression of Rb protein, and Rb cDNA could be found in the transfected cells. The proliferation of transfected CAOV3 cells was inhibited evidently. Sixty-eight per cent of cells were arrested at G(0), G(1) phases of cell cycle. CONCLUSIONS: The recombinant Rb gene adenovirus vector could transform CAOV3 cells efficiently and inhibit CAOV3 cell growth. It might be a new approach of gene therapy for ovarian cancer.