喜马拉雅土拨鼠非冬眠期乳酸脱氢酶同工酶基因表达特征

用聚丙烯酰胺凝胶电泳和紫外光谱法分析非冬眠期喜马拉雅土拨鼠4种组织的乳酸脱氢酶(LDH)同工酶的酶谱及其活力,该鼠骨骼肌酶带的多态分布,可能是潜在的调节基因调控所致。另外,本文还对构象异构体产生的亚带进行了研讨。

Characterization of the Expression of LDH Isozymes Genes During Non-hibernation Himalayan Marmot

The lactate dehydrogenase (LDH) isozymes from heart, liver, kidney and skeletal muscle in Himalayan marmot (Marmota himalayana robusta) in non-hibernation were investigated in the present experiment. The isozyme patterns were analyzed by means of disc eletr-ophoresis and the ultraviolet enzymatic determination. The following conclusions were obtained:1. It was found that two expression patterns of LDH genes presented in the skeletal muscle of Himalayan marmot. There was a LDH-B4 band only in one marmot and absent in others. The interinvidual polymorphism of LDH isozyme patterns may be attribute to one or more regulator genes controlling synthesis of B-subunit of LDH.2. LDH zymograms of various tissues of Himalayan marmot showed different subbands. This is presumably due to the existence of both symmetrical and asymmetrical confermers of this tetramer.