Skp2和p27在卵巢上皮性肿瘤组织中的表达和意义

目的:探讨卵巢上皮性肿瘤组织中Skp2和p27表达的意义以及Skp2和p27表达的相关性。方法:用免疫组化法分别检测卵巢腺瘤(n=20),交界性卵巢腺瘤(n=20),卵巢腺癌(n=50)标本Skp2和p27的表达。以年龄、病理分级、临床分期、病理类型和淋巴结转移作为卵巢腺癌的独立因素进行分析。结果:卵巢腺癌Skp2表达率与卵巢腺瘤和交界性卵巢腺瘤相比显著增高(P<0.01,P<0.01)。卵巢腺癌p27表达率与卵巢腺瘤和交界性卵巢腺瘤相比显著降低(P<0.01,P<0.01)。卵巢腺癌病理分级为Ⅱ~Ⅲ级者Skp2表达显著高于Ⅰ级者(P<0.05),临床分期Ⅲ~Ⅳ期者显著高于Ⅰ~Ⅱ期者(P<0.001)。卵巢腺癌病理分级为Ⅱ~Ⅲ级者p27表达显著低于Ⅰ级者(P<0.01),临床分期Ⅲ~Ⅳ期者显著低于Ⅰ~Ⅱ期者(P<0.05)。与无淋巴结转移者比较,有淋巴结转移者Skp2表达率较高(P<0.001),p27表达较低(P<0.05)。结论:卵巢腺癌Skp2呈高表达和p27呈低表达,卵巢腺癌中Skp2和p27表达呈负相关。Skp2的高表达和p27的低表达分别在人卵巢上皮癌的发生,发展中可能起一定的作用,并与预后相关。

Skp2 and p27 expression in human epithelial ovarian tumor

Objective:To investigate the expression of Skp2 and p27 and their relationship in human epithelial ovarian tumor.Methods: The specimens of patients suffering from adenoma (n=20),borderline adenoma(n=20) and adenocarcinoma (n=50) were detected by immunohistochemical method for exploring the expression of skp2 and p27.The findings were analyzed in terms of age group,pathological grade,clinical stage,histological type and the state of lymph node metastasis.Results: Skp2 labeling frequency was increased dramatically in adenocarcinoma compared with adenoma and borderline adenoma (P<0.01,P< 0.01 ),while p27 labeling frequency was significantly lower than that of adenoma and borderline adenoma (PP<0.01).In adenocarcinoma,Skp2 labeling frequency in pathologic grade Ⅱ～Ⅲ was evidently higher than that of pathologic grade Ⅰ(P<0.05),and clinical stage Ⅲ～Ⅳ presented prominent compared with clinical stage Ⅰ～Ⅱ (P<0.001).The expression of p27 in pathologic grade Ⅱ～Ⅲ marked lower than that in pathologic gradeⅠ(P<0.01),and clinical stage Ⅲ～Ⅳ was less frequent in comparison with clinical stageⅠ～Ⅱ(P<0.05).Specimens with lymph node metastasis showed a higher expression of skp2(P< 0.001 ) and a lower expression of p27(P<0.05) compared with those with no metastasis.Conclusion:The inverse interaction of Skp2 and p27 may correlate with carcinogenesis and progression of human epithelial ovarian adenocarcinoma.