| 高效液相色谱法测定丹参中丹酚酸B |
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| 引用本文： | 张启伟,张颖,李计萍,马华.高效液相色谱法测定丹参中丹酚酸B[J].中国中药杂志,2001,26(12):848-849. |
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| 作者姓名： | 张启伟 张颖 李计萍 马华 |
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| 作者单位： | 中国中医研究院中药研究所, |
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| 基金项目： | 国家重点科技项目专题(96-903-01-02) |
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| 摘 要： | 目的 :建立丹参中丹酚酸B高效液相色谱测定方法。方法 :75%甲醇回流提取 ,色谱柱YWGC18,流动相甲醇 5%乙酸 (35∶65 ) ,检测波长281nm。结果 :丹酚酸B0.17～1.70μg线性关系良好 (r =0.9997) ,回收率98.9% ,RSD1.82%。结论 :为丹参及其制剂的质量控制提供了分析方法。
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| 关 键 词： | 丹参 丹酚酸B 含量测定 高效液相色谱法 |
| 文章编号： | 1001-5302(2001)12-0848-02 |
| 收稿时间： | 7/3/2000 12:00:00 AM |
| 修稿时间： | 2000年7月3日 |
Determination of Salvianolic Acid B in the Radix of Salvia miltiorrhiza Bge. by HPLC |
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| ZHANG Qi wei;ZHANG Ying;LI Ji ping;MA Hua.Determination of Salvianolic Acid B in the Radix of Salvia miltiorrhiza Bge. by HPLC[J].China Journal of Chinese Materia Medica,2001,26(12):848-849. |
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| Authors: | ZHANG Qi wei;ZHANG Ying;LI Ji ping;MA Hua |
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| Affiliation: | Institute of Chinese Materia Medica, China Academy of TCM, Beijing 100700, China. |
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| Abstract: | OBJECTIVE: To develop an HPLC method for determining salvianolic acid B in radix of Salvia miltiorrhiza. METHOD: The sample was extracted with 75% methanol. ODS column was used with methanol-5% acetic acid solution (35:65) as mobile phase. Detection wavelength was 281 nm. RESULT: Salvianolic acid B was separated well. Good linearity of salvianolic acid B was obtained (r = 0.9997) within the range of 0.17-1.7 micrograms. The average recovery was 98.9%. Repeatability was good, and RSD was 1.82%. CONCLUSION: This method can be used for quality control of radix of Salvia miltiorrhiza. |
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| Keywords: | radix of Salvia miltiorrhiza salvianolic acid B quantitative determination HPLC |
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