| 高效液相色谱法测定胃灵胶囊中芍药苷含量 |
| |
| 引用本文： | 王芳,胡雪莲,陈琳,雷健,余涛,曹健.高效液相色谱法测定胃灵胶囊中芍药苷含量[J].中国药业,2011,20(1):17-18. |
| |
| 作者姓名： | 王芳 胡雪莲 陈琳 雷健 余涛 曹健 |
| |
| 作者单位： | 第三军医大学新桥医院药学部,重庆,400037 |
| |
| 摘 要： | 目的建立测定胃灵胶囊中芍药苷含量的高效液相色谱法。方法采用Hypersil ODS色谱柱(150 mm×4.6 mm,10μm),流动相为乙腈-0.1%磷酸溶液(15∶85),检测波长为229 nm,流速1 mL/min,柱温30℃。结果芍药苷质量浓度在5.1～25.5μg/mL范围内与峰面积线性关系良好,r=0.999 9(n=5);平均回收率为97.30%,RSD=3.02%(n=9)。结论高效液相色谱法重现性好、简便易行,可用于胃灵胶囊的质量控制。
|
| 关 键 词： | 高效液相色谱法 胃灵胶囊 芍药苷 含量测定 |
Determination of Paeoniflorin in Weiling Capsules by HPLC |
| |
| Wang Fang,Hu Xuelian,Chen Lin,Lei Jian,Yu Tao,Cao Jian.Determination of Paeoniflorin in Weiling Capsules by HPLC[J].China Pharmaceuticals,2011,20(1):17-18. |
| |
| Authors: | Wang Fang Hu Xuelian Chen Lin Lei Jian Yu Tao Cao Jian |
| |
| Affiliation: | Wang Fang,Hu Xuelian,Chen Lin,Lei Jian,Yu Tao,Cao Jian(Department of Pharmacy,Xinqiao Hospital,Third Military Medical University,Chongqing,China 400037) |
| |
| Abstract: | Objective To establish an HPLC method for the determination of paeoniflorin in Weiling Capsules.Methods To use the Hypersil ODS column（150 mm×4.6 mm,10 μm）,the mobile phase was acetonitrile-0.1%phosphoric acid（15 ∶85）,the detection wavelength was 229 nm,the flow rate was 1 mL/min and the column temperature was 30 ℃.Results The linear range of paeoniflorin was 5.1-25.5 μg/mL,r=0.999 9.The average recovery rate was 97.30%,RSD was 3.02%（n=9）.Conclusion The method was simple and accurate,which can be used for the quality control of Weiling Capsules. |
| |
| Keywords: | HPLC Weiling Capsules paeoniflorin content determination |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
