哈茨木霉对水稻恶苗病菌的拮抗作用

PDA平板拮抗试验表明 ,哈茨木霉对水稻恶苗病菌有强烈的拮抗作用 ,其孢子悬浮液的含孢量为 10⁶～10⁷个 /mL时 ,对恶苗病菌的抑制力达 92.33%。通过哈茨木霉菌液和 3种药剂对水稻恶苗病菌抑制效果的比较 ,哈茨木霉孢子悬浮液含孢量为 10⁷个 /mL与施保克质量浓度 1μg/mL的抑菌效果接近 ,分别为 76.7%、75.4%。显微摄影结果显示 ,哈茨木霉以附着胞附着在恶苗病菌菌丝上 ,然后穿透菌丝在其内生长 ,或与恶苗病菌的菌丝平行生长 ,然后再侵入病菌内寄生。

Antagonism of Trichoderma harzianum against Fusarium moniliforme on rice

The antagonistic experiment on PDA showed that Trichoderma harzianum had strong antagonism against Fusarium moniliforme. The effective concentration was at 10⁶～10⁷ cfu/mL, and the inhibitory efficacy reached 92.33% . Through another measuring experiment of T. harzianum and three kinds of fungicides against F. moniliforme, a conclusion could be drawn: the concention of 10⁷ cfu/mL of T. harzianum had almost the same effect as 1 μg/mL of sportak. The inhibition ratios were 76.7% and 75.4%, respectively. By microscopy photograph, the investigations showed that T. harzianum could either adhere to the hyphae of F. moniliforme with appressoria or grow parallel with the hyphae of E. moniliforme, then penetrated and parasitized in them.