乌药挥发油对寒凝气滞血瘀证大鼠血液流变学及NO-sGC-cGMP信号通路的影响

目的 探讨乌药挥发油对寒凝气滞血瘀证大鼠中医证候积分、血液流变学及一氧化氮（nitric oxide,NO）-可溶性鸟苷酸环化酶（soluble guanylate cyclase,sGC）-环磷酸鸟苷（cyclic guanosine monophosphate,cGMP）信号通路的影响。方法 将48只SD雄性大鼠随机分为对照组、模型组及乌药挥发油高、中、低剂量（3.76、1.88、0.94 g/kg）组和复方丹参片（1.20 g/kg）组，每组8只。除对照组外，其余组大鼠采用sc肾上腺素加冰水浴双因素复合刺激14 d制备寒凝气滞血瘀证模型。从造模第15天开始，各给药组ig相应药物，对照组和模型组ig等体积2%聚山梨酯-80溶液，1次/d，连续7 d；在给药过程中间隔2 d给予1次造模条件，维持大鼠血瘀证表现。末次给药后的第2天对各组大鼠进行中医证候评分，测定各组大鼠血液流变学及血浆NO、内皮素（endothelin,ET）水平；采用苏木素-伊红（HE）染色法观察胸主动脉组织形态；采用ELISA法检测胸主动脉组织中环磷酸腺苷（cyclic adenosine monophosphat...

Effect of volatile oil from Linderae Radix on hemorheology and NO-sGC-cGMP signaling pathway in rats with qi stagnation and blood stasis syndrome

Objective To observe the effect of volatile oil from Wuyao (Linderae Radix) on hemorheology and nitric oxide (NO)-soluble guanylate cyclase (sGC)-cyclic guanosine monophosphate (cGMP) signaling pathway in rats with cold qi stagnation and blood stasis syndrome (QSBSS). Methods Forty-eight SD male rats were randomly divided into control group, model group, volatile oil from Linderae Radix high-, medium-, low-dose (3.76, 1.88, 0.94 g/kg) groups and Fufangdanshen tablets (1.2 g/g) group, with eight rats in each group. Except control group, rats of QSBSS model were prepared by two-factor stimulation of sc adrenaline plus ice water bath for 14 d. Starting from the 15th day of modeling, each administration group was ig corresponding drug, control group and model group were ig 2% tween-80 solution, once a day for 7 d. During the administration process, the modeling condition was given once at an interval of 2 d to maintain the manifestation of blood stasis in rats. On the 2nd day after the last administration, rats in each group were scored for TCM syndromes, and hemorheology, plasma NO and endothelin (ET) levels of rats in each group were determined; Hematoxylin-eosin (HE) staining was used to observe the morphology of thoracic aorta; Levels of cyclic adenosine monophosphate (cAMP) and cGMP in thoracic aortic tissues were determined by ELISA; Phosphodiesterase 5A (PDE5A) and sGC protein expressions in thoracic aortic tissues were detected by Western blotting. Results Compared with control group, TCM syndrome score, blood rheology (whole blood viscosity, plasma viscosity, erythrocyte aggregation index, fibrinogen), plasma ET level, PDE5A protein expression in thoracic aorta were significantly higher in model group (P < 0.01), plasma NO levels, cAMP and cGMP contents, sGC protein expression in thoracic aorta were significantly lower (P < 0.01). Compared with model group, rats in volatile oil from Linderae Radix group showed lower TCM syndrome score, blood rheological indexes, plasma ET levels, PDE5A protein expression in thoracic aorta (P < 0.05, 0.01), while significantly higher plasma NO levels, cAMP and cGMP contents, sGC protein expression in thoracic aorta (P < 0.05, 0.01). HE staining of rat thoracic aorta showed that vascular intima of rats in model group was significantly damaged, vascular endothelial cells were incomplete, and vascular smooth muscle cells showed spiral contraction-like changes. The damage of vascular intima and vascular endothelial cells, contraction of vascular smooth muscle were significantly improved in volatile oil from Linderae Radix group. Conclusion Volatile oil from Linderae Radix can effectively improve the TCM syndrome score and blood rheology in rats with QSBSS, and exert the effect of vascular endothelial cell protection and improving blood hypercoagulation, and its mechanism is related to the regulation of NO-sGC-cGMP signaling pathway.