| 离体条件下进行治疗枣疯病药物筛选的可行性研究 |
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| 引用本文： | 赵锦,代丽,薛陈心,刘孟军.离体条件下进行治疗枣疯病药物筛选的可行性研究[J].河北农业大学学报,2006,29(1):70-73. |
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| 作者姓名： | 赵锦 代丽 薛陈心 刘孟军 |
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| 作者单位： | 1. 河北农业大学,生命科学学院,河北,保定,071000
2. 河北农业大学,中国枣研究中心,河北,保定,071001 |
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| 基金项目： | 国家自然科学基金项目(39970523)，河北省自然基金项目(300129) |
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| 摘 要： | 以婆枣带枣疯病组培苗为试材,在培养基中添加不同浓度及不同次数的盐酸-四环素(TETRACYCLINE,TC)和盐酸-土霉素(OXYTETRACYCLINE,OX)药物,培养40 D后,观察其对病苗生长情况的影响,并对转健苗进行植原体特异PCR检测,结果表明,25ΜG/ML的药物浓度处理一次可使枣疯病症状消失,并完全杀灭枣疯病病原,达到理想效果;同时本研究中田间和组培条件下相同药物治疗效果的一致性进一步说明了利用组织培养技术进行药物筛选的可行性。
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| 关 键 词： | 枣疯病 组培苗 植原体 药物 |
| 文章编号： | 1000-1573（2006）01-0070-04 |
| 收稿时间： | 2005-06-29 |
| 修稿时间： | 2005-06-29 |
Selecting remedial drugs for witches' broom disease via tissue culture |
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| ZHAO Jin,DAI Li,XUE Chen-xin,LIU Meng-jun.Selecting remedial drugs for witches'''' broom disease via tissue culture[J].Journal of Agricultural University of Hebei,2006,29(1):70-73. |
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| Authors: | ZHAO Jin DAI Li XUE Chen-xin LIU Meng-jun |
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| Affiliation: | 1. College of Life Science, Agricultural University of Hebei, Baoding 071000, China; 2. Research Center of Chinese Jujube, Agricultural University of Hebei, Baoding 071001, China |
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| Abstract: | The plantlets with witches' broom disease of Ziziphus jujuba cv.Pozao were used as materials in this paper.The diseased plantlets were cultured in the medium with different concentration and times of Tc and Ox.After 40 days,the growth conditions of the treated plantlet were observed and the phytoplasma of healing plantlets was also detected by PCR.The results showed that 25 μg/mL Tc and Ox could reverse the diseased symptom and kill phytoplasma completely.At the same time,the consistency of treated effect between field and tissue culture also showed that selecting remedial drugs via tissue culture was feasible. |
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| Keywords: | witches' broom disease drugs phytoplasma plantlet |
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