| α-环糊精葡萄糖基转移酶菌株的筛选与鉴定 |
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| 引用本文： | 张智维,雷新辉,张海群.α-环糊精葡萄糖基转移酶菌株的筛选与鉴定[J].西北轻工业学院学报,2012(6):89-92. |
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| 作者姓名： | 张智维 雷新辉 张海群 |
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| 作者单位： | 陕西科技大学生命科学与工程学院 |
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| 基金项目： | 陕西省自然基金项目(2009JM2005);陕西省教育厅专项科研计划项目(09JK372) |
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| 摘 要： | 从富含淀粉的土样中，初筛出22株产α-环糊精葡萄糖基转移酶菌株，然后用透明圈法筛选出3株酶活力较高的菌株，最后绘制酶活力曲线，得到一株酶活力为0．64U／mL的菌株N1．通过菌落形态、显微观察和生理生化特性，鉴定N1为肠杆菌科的产酸克雷伯氏菌．
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| 关 键 词： | α-环糊精葡萄糖基转移酶 筛选 鉴定 酶活力 |
Selecting and identification of the producing strain for α-Cyclodextrin Glycosyltransferase |
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| ZHANG Zhi-wei,LEI Xin-hui,ZHANG Hai-qun.Selecting and identification of the producing strain for α-Cyclodextrin Glycosyltransferase[J].Journal of Northwest University of Light Industry,2012(6):89-92. |
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| Authors: | ZHANG Zhi-wei LEI Xin-hui ZHANG Hai-qun |
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| Affiliation: | (Colege of Life Science and Engineering,Shaanxi University of Science & Technology,Xi′an 710021,China) |
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| Abstract: | 22 strains of bacteria which could yield α-CGTase were prescreened from soil sire ples. These soils are rich in starch. 3 strains of them with higher enzymatic activity were further selected by assaying transparent zone. Finally, we obtained a strain with activity of 0.86 U/mL from setting up a standard curve of enzymatic activity. This research showed that N1 was a Klebsiella oxytoca based on its colony morphology, microscopic observation and physiology and biochemical characteristic. |
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| Keywords: | α-Cyclodextrin Glyeosyltransferase selection identification enzyme activity |
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