多环芳烃荧蒽诱导拟南芥氧化胁迫

选用模式植物拟南芥为材料，以四环的多环芳烃（PAHs）荧蒽为研究对象，从植物对非生物胁迫响应紧密相关的抗氧化酶及膜保护系统的变化入手，研究了植物对多环芳烃胁迫的生理响应.结果表明：荧蒽胁迫下拟南芥经历了氧化胁迫和膜脂过氧化过程.0.75 mmol·L^-1的荧蒽使拟南芥光合作用过程受到抑制；1.00 mmol·L^-1的荧蒽使拟南芥丙二醛(MDA)含量极显著增加, 抗坏血酸过氧化物酶(APX)活性极显著下降, 膜脂过氧化作用加剧，1.25 mmol·L^-1的荧蒽使拟南芥过氧化物酶(POD)活性极显著下降，H₂O₂在细胞内累积，拟南芥明显受害.

Responses of Arabidopsis thaliana to oxidative stress induced by polycyclic aromatic hydrocarbon fluoranthene.

With the seedlings of model plant Arabidopsis thaliana and the polycyclic aromatic hydrocarbon fluoranthene as test materials, this paper studied the physiological responses of plants to polycyclic aromatic hydrocarbons from the aspects of antioxidases and membrane protection system that are tightly related to abiotic stresses. The results showed that under fluoranthene stress, the seedlings experienced oxidative stress and membrane-lipid peroxidization. At 0.75 mmol x L(-1) of fluoranthene, the photosynthetic process was inhibited; at 1.00 mmol x L(-1) of fluoranthene, the malonaldehydic acid (MDA) content increased and the ascorbate peroxidase (APX) activity decreased significantly, indicating an accelerated membrane-lipid peroxidization; and at 1.25 mmol x L(-1) of fluoranthene, the peroxidase (POD) activity decreased significantly and H2O2 had a high accumulation, making the seedlings injured severely.