葡萄糖酸镁对大鼠心肌缺血再灌注损伤的保护作用

目的: 观察葡萄糖酸镁对心肌缺血再灌注损伤的影响, 探讨葡萄糖酸镁心肌保护作用及其机理。方法: 采用改进了的整体大鼠急性心肌缺血再灌注损伤模型, 检测组织内 Na⁺-K⁺ -ATPase 活力及丙二醛(MDA) 和 Ca²⁺ 含量, 并测定由ADP 诱导的血小板5 min 最大聚集率 AGG(M)。结果: 与假手术组相比, 缺血再灌注组心肌组织内 Na⁺ -K⁺ -ATPase 活力显著下降(P<0.01), MDA 和Ca²⁺ 含量明显升高(P<0.01), AGG(M) 升高(P<0.05)。与缺血再灌注组比较, 葡萄糖酸镁 3 个不同剂量保护组心肌组织内Na⁺ -K⁺-ATPase 活力明显升高(P<0.01), MDA和Ca²⁺ 含量明显降低(P<0.05 或 P <0.01), AGG(M) 也明显降低(P<0.01), 且各作用均随用药剂量的增加而更加显著。结论: 葡萄糖酸镁对心肌缺血再灌注损伤有保护作用, 其保护作用机制与增加Na⁺ -K⁺-ATPase 活性 、减轻钙超载和抑制脂质过氧化反应以及血小板聚集有关。

Cardioprotective effects of magnesium gluconate on myocardial ischemia reperfusion injury in rats

AIM: To observe the effects of magne-sium gluconate on myocardial ischemia reperfusion injury in anesthetized rats and to investigate the cardioprotective effects of magnesium gluconate. METHODS: The im-proved model of myocardial ischemia reperfusion injury in anesthetized ratswas adopted. Na⁺-K⁺ -ATPase activity, malondialdehyde (MDA) and Ca²⁺ contents in myocardi-um were assayed.The platelet aggregation AGG(M) and Mg²⁺ in blood serum were assayed at the same time. RESULTS: Na⁺ -K⁺ -ATPase activity decreased and MDA and Ca²⁺ contents increased in the ischemia reperfusion group compared with sham.The AGG(M) and Na⁺ -K⁺ ATPase activity increased, and MDA and Ca²⁺ contents and AGG (M) decreased in the three different dosages group of magnesium gluconate comparedwith the ischemia reperfusion group.The cardioprotective effects increased with the dose increasing of magnesium gluconate. CONCLUSION: Magnesium gluconate has protective effects on myocardial ischemia reperfusion injury in rats.It may be related to increasing the activity of Na⁺ -K⁺ -ATPase, and to relieving calcium overload and inhibiting lipid per-oxidation and platelet aggregation.