| Abstract: | Bioreactors with covalently supported ionic liquid phases (SILP) were prepared as polymeric monoliths based on styrene–divinylbenzene or 2‐hydroxyethyl methacrylate–ethylene dimethacrylate, and with imidazolium units loadings ranging from 54.7 to 39.8 % wt IL per gram of polymer. The SILPs were able to absorb Candida antarctica lipase B (CALB), leading to highly efficient and robust heterogeneous biocatalysts. The bioreactors were prepared as macroporous monolithic mini‐flow systems and tested for the continuous flow synthesis of citronellyl propionate in supercritical carbon dioxide (scCO2) by transesterification. The catalytic activity of these mini‐flow‐bioreactors remained practically unchanged for seven operational cycles of 5 h each in different supercritical conditions. The best results were obtained when the most hydrophobic monolith, M‐SILP‐ 8 ‐CALB, was assayed at 80 °C and 10 M Pa, reaching a total turnover number (TON) of 35.8×104 mol product/mol enzyme. The results substantially exceeded those obtained for packed‐bed reactors with supported silica‐CALB‐Si‐4 catalyst under the same experimental conditions. |
