烟草青枯病抗性与分子标记的关联分析

为了找到与烟草青枯病抗性相关的分子标记、加快抗病品种的选育,以78份烟草种质为自然群体材料,选用20对简单重复序列(Simple Sequence Repeat,SSR)和37对微卫星锚定片段长度多态性(Microsatellite-anchored Fragment Length Polymorphism,MFLP)引物组合对烟草种质材料进行基因分型,并利用等位变异数据进行主成分分析和群体结构分析,同时采用TASSEL3.0软件对烟草青枯病抗性与等位变异进行连锁不平衡关联分析。结果显示:SSR和MFLP标记在78份烟草种质中共检测到252个等位变异,主成分分析和群体结构分析均将烟草种质分为5个组群;关联分析发现6个MFLP标记位点与烟草青枯病抗性显著相关,对青枯病抗性的解释率在8.33%~19.49%之间。这些分子标记可为评价具有青枯病抗性潜力的烟草种质材料提供依据。 

Association Analysis of Tobacco Bacterial Wilt Resistance with Molecular Markers

In order to find out the molecular markers related to tobacco bacterial wilt(TBW) resistance and speed up the breeding process of cultivars resistant to TBW,78 tobacco accessions were selected as natural population,genotyping was performed with 20 pairs of simple sequence repeat(SSR) primers and 37 pairs of microsatellite-anchored fragment length polymorphism(MFLP) primers.Principal component analysis and population structure analysis were conducted with allelic variation data,and the linkage disequilibrium association analysis between TBW resistance and allelic variants was carried out by TASSEL3.0 software.The results showed that 252 allelic variants were detected by SSR and MFLP in 78 tobacco accessions,these accessions were classified into 5 groups by both principal component analysis and population structure analysis.Association analysis indicated that 6 MFLP loci significantly associated with TBW resistance,which explained 8.33%-19.49% of the phenotypic variation of TBW resistance.These molecular markers can be used as reference indexes for evaluating the tobacco accessions with potential TBW resistance.