RP—HPLC法同时测定枇杷茎枝、叶中齐墩果酸和熊果酸的含量

目的：建立不同产地枇杷中齐墩果酸、熊果酸的含量测定方法。方法：采用RP—HPLC法，齐墩果酸、熊果酸采用乙醇超声提取30分钟，用Agilent Eclipse XDB—C18柱，甲醇-乙腈-0．05％醋酸铵溶液（12：67：21）为流动相，流速1．0ml／min，检测波长205nm。结果：齐墩果酸和熊果酸的线性范围分别为2．61—209mg．L^-1（r=0．9998），7．85—628mg．L^-1（r=0.9999），与峰面积呈良好线性关系，平均加样回收率依次为97．27％，99．75％。结论：本方法操作简单，结果准确，重现性好，适用于琵琶中齐墩果酸、熊果酸含量测定。

Determination of oleanolic acid and ursolic acid in branch and leaf of Eriobotrya Japonica by HPLC

Objective： To establish a method for determination of oleanolic acid and ursolic acid in Eriobotrya Japonica from different habitats by HPLC. Method： Oleanolic acid and ursolic acid was extracted with ethanol solution by ultrasound for 30 minutes, and the content of berberine was determined by RP - HPLC. Agilent C18 column （250 mm ×4. 6 mm, 5 μm） was used for the determination, with a flow rate of 1. 0 mL · min - 1 and column temperature of 25℃. The mobile phase were methanol - acetonitrile - 0. 05% solution of ammonium acetate （ 12 ： 67 ： 21 ） and detection wave length was 205nm. Result： There was good linearity in the range of 2. 61 -209mg. L^-1 （r=0. 9998）, 7. 85-628mg. L^-1 （r=0. 9999） ofoleanolic acid and ursolic acid. Average recovery rate was 97. 27%, 99. 75%. Conclusion： The method is simple, reliable and repeatable, and is suitable for the determination of oleanolic acid and ursolic acid in Eriobotrya Japonica.