| 去氢骆驼蓬碱诱导急性白血病Jurkat细胞凋亡及其机制 |
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| 引用本文： | 刘俊玲,乌庆超,王朝晖,洪青,王九河,韩波,范传波,田猛. 去氢骆驼蓬碱诱导急性白血病Jurkat细胞凋亡及其机制[J]. 光明中医, 2012, 27(2): 242-244 |
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| 作者姓名： | 刘俊玲 乌庆超 王朝晖 洪青 王九河 韩波 范传波 田猛 |
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| 作者单位： | 青岛市海慈医疗集团血液科,青岛,266033 |
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| 基金项目： | 山东省科技发展计划项目（No.Kzd-60） |
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| 摘 要： | 目的本研究旨在研究去氢骆驼蓬碱体外诱导Jurkat细胞凋亡及其可能机制。方法采用MTT法测定去氢骆驼蓬碱对Jurkat细胞的生长抑制作用;用光学显微镜检、琼脂糖凝胶电泳检测去氢骆驼蓬碱诱导Jurkat细胞凋亡;应用蛋白印迹法检测去氢骆驼蓬碱诱导Jurkat细胞凋亡过程中bcl-2与ICAD的表达变化情况。结果去氢骆驼蓬碱对Jurkat细胞有明显的生长抑制作用,其增殖抑制作用与诱导细胞凋亡有关;bcl-2与ICAD在Jurkat细胞中均有表达,且随药物浓度的增高表达量逐渐降低。结论去氢骆驼蓬碱可诱导Jurkat细胞凋亡,bcl-2与ICAD在去氢骆驼蓬碱诱导Jurkat细胞凋亡的信号传导途径中可能是重要的调控因子。
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| 关 键 词： | 去氢骆驼蓬碱 Jurkat细胞 细胞凋亡 |
Harmine - Inducing Apoptosis of Jurkat Leukemia Cells and Its Mechanism in Vitro |
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| Affiliation: | Liu Junling,Wu Qingchao,Wang Zhaohui, et al. ( Department of Hematology, Qingdao Hiser Medical Center, Qingdao 266033, China) |
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| Abstract: | Objective：The study was aimed to investigate the effect of harmine （ HM ） on the apoptosis of Jurkat leukemia cells and its mechanism in vitro. Methods：The inhibition of HM on Jurkat ceils were evaluated by means of MTT assay; cells apoptosis was detected by light microscopy , agarose gel electrophoresis ; Western blot was used to analyze the expression of bcl - 2 and ICAD in cells during apoptosis induced by HM. Results： showed that HM could significantly inhibit the proliferation of Jurkat cells, and its inhibition effect contributed to the induced apoptosis, bcl - 2 and ICAD proteins both all expressed in Jurkat cells , the level of expression declined as concentration increased. Conclusion ： HM may induced apoptosis of Jurkat cells in vitro, bcl -2 and ICAD may be an important control factor in thesignal transduction pathway of HM - induced apoptosis. |
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| Keywords: | harmine Jurkat cells apoptosis |
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