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时间分辨在检测抗-HBs的优势应用
引用本文:娄宏哲.时间分辨在检测抗-HBs的优势应用[J].中国实用医药,2010,5(18):51-52.
作者姓名:娄宏哲
作者单位:沈阳市胸科医院,110041
摘    要:目的定量检测人体抗-HBs对乙肝免疫的重要性。方法用时间分辨的方法(TRFIA)、酶联免疫吸附实验(ELISA)的方法以及胶体金免疫层析(GICA)的方法比对三者结果的符合率。结果时间分辨的方法和ELISA与胶体金免疫层析的方法阳性吻合率分别为98.6%和97.7%。结论ELISA和胶体金方法只能测量乙肝抗-HBs阴性阳性结果,但对设备要求简单,回报结果迅捷,适用于大量标本的初筛;而时问分辨给出了抗-HBs的量化结果,更容易动态观察乙肝的病情发展以及疫苗注射情况。

关 键 词:时间分辨  酶联免疫吸附实验  胶体金  抗-HBs疫苗注射

Time-resolved detection of the advantages of application of anti-HBs
LOU Hong-zhe.Time-resolved detection of the advantages of application of anti-HBs[J].China Practical Medical,2010,5(18):51-52.
Authors:LOU Hong-zhe
Affiliation:LOU Hong-zhe. Shenyang Chest Hos- pital Laboratory Medicine, Liaoning , Shenyang 110041 China
Abstract:Objective Quantitative detection of human anti-HBs on the importance of hepatitis B immunization. Methods Compared the resut time-resolved method (TRFIA) , enzyme-linked immunosorbent as- say (ELISA) method, and colloidal gold immunochromatography assay (GICA). Results Time-resolved meth- od and ELISA and colloidal gold immunochromatographic method of positive consistent rate was 98. 6% and 97.7%. Conclusion ELISA and colloidal gold method can only measure of anti-HBs negative HBV positive results, but the device requires is a simple, quick return results for screening a large number of specimens;and time-resolved given the quantitative results of anti-HBs is easier dynamic observe the progression of the disease, as well as hepatitis B vaccination of.
Keywords:Time-resolved  Enzyme-linked immunosorbcnt assay  Colloidal gold  Anti-HBs Vaccination
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