清肠泡腾栓调控溃疡性结肠炎细胞因子的实验研究 |
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引用本文: | 陈英群,马贵同,冯怡.清肠泡腾栓调控溃疡性结肠炎细胞因子的实验研究[J].中国中西医结合杂志,2005(Z1). |
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作者姓名: | 陈英群 马贵同 冯怡 |
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作者单位: | [1]同济大学附属第十人民医院中医科三病区 [2]上海中医药大学龙华医院消化科 [3]上海中医药大学中药学院 上海 |
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基金项目: | 上海市科委2003年博士发展基金 |
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摘 要: | 目的探讨中药肠道泡腾栓剂治疗溃疡性结肠炎的免疫学疗效机制。方法实验分设正常对照、模型对照、空白泡腾栓、清肠泡腾栓、清肠栓、柳氮磺胺吡啶栓共6个组,ELISA法检测血清、结肠上清TNF-α仪、IL-13;免疫组化、原位杂交检测IL-8(mRNA)阳性表达。结果模型对照组血清TNF-α较正常对照组升高(P<0.05),结肠黏膜IL-8(mRNA)阳性表达明显增强(P<0.01);清肠泡腾栓明显降低结肠上清TNF-α含量以及IL-8(mRNA)阳性表达,与模型组比较差异有显著性(P<0.05,P<0.01);清肠泡腾栓对IL-13的调控作用不明显,与模型对照组比较差异无显著性(P>0.05)。结论清肠泡腾栓通过抑制促炎细胞因子而对溃疡性结肠炎产生治疗作用。
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关 键 词: | 清肠泡腾栓 溃疡性结肠炎 TNF-α IL-8 IL-13 实验研究 |
Experimental Study of Qingchang Effervescent Suppository in Regulating Ulcerative Colotitis Cytokines |
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Authors: | CHEN Ying-qun MA Gui-tong Feng Yi |
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Abstract: | Objective To observe the immunity therapeutic mechanism of Qingchang Effervescent Supposito- ry(QES)in treating ulcerative colotitis(UC).Methods Six groups were set up,i.e.the normal control,the mod- el,the blank effervescent suppository(BES),QES,sulfasalazine(SASP)suppository and Qingchang suppository gronps.Tumor necrosis factorα(TNF-α)and IL-13 in blood serum and colon supernate were tested by ELISA,and interleukin-8(IL-8)mRNA positive expression by immunohistological method and in situ hybridization.Res... |
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Keywords: | Qingchang Effervescent Suppository ulcerative colotitis tumor necrosis factorα interleukin-8 interleukin-13 experimental study |
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