| 兔感觉神经特异蛋白的纯化及稳定性观察 |
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| 引用本文： | 蒲小平,汪家政,张杰,顾晓松,范明.兔感觉神经特异蛋白的纯化及稳定性观察[J].生物化学与生物物理进展,1997,24(5):465-466. |
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| 作者姓名： | 蒲小平 汪家政 张杰 顾晓松 范明 |
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| 作者单位： | 军事医学科学院基础医学研究所，北京 100850;军事医学科学院基础医学研究所，北京 100850;军事医学科学院基础医学研究所，北京 100850;南通医学院神经科学研究所，南通 226001;军事医学科学院基础医学研究所，北京 100850 |
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| 基金项目： | 国家自然科学基金(39470156)和国家教委留学回国人员科研启动基金(1996-644)资助. |
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| 摘 要： | 以兔脊神经节及背根纤维为材料,通过制备匀浆,DEAE-Sephacel阴离子交换层析,高压液相凝胶过滤层析分离纯化了感觉神经特异蛋白29 ku,并进行了该蛋白的稳定性观察.
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| 关 键 词： | 感觉神经特异蛋白 纯化 稳定性 |
| 收稿时间： | 4/9/1997 12:00:00 AM |
| 修稿时间： | 1997/7/11 0:00:00 |
Purification and Stability Observation of Sensory Nerve-specific Protein in Rabbit |
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| PU Xiaoping,WANG Jiazheng,ZHANG Jie,GU Xiaosong and FAN Ming.Purification and Stability Observation of Sensory Nerve-specific Protein in Rabbit[J].Progress In Biochemistry and Biophysics,1997,24(5):465-466. |
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| Authors: | PU Xiaoping WANG Jiazheng ZHANG Jie GU Xiaosong and FAN Ming |
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| Affiliation: | Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China;Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China;Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China;Nantong Medical College, Nantong 226001, China;Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China |
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| Abstract: | A 29 ku protein, the sensory nerve-specific protein was isolated and purified from rabbit spinal ganglion and its fibers by anion exchange chromatography using DEAE-Sephacel and gel filtration HPLC. The purity was extiomatd to be over 80% by UV scanning. Western blot demonstrated that it immunoreacts with anti-rabbit the 29 ku protein monoclonal antibody. The stability also has been observed. |
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| Keywords: | sensory nerve-specific protein purification stability |
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