平行定量浓缩仪- 超高效液相色谱- 串联质谱联用快速检测鳗鱼中8 种喹诺酮类药物残留量

建立鳗鱼肉中8种奎诺酮残留量的平行定量浓缩仪-超高效液相色谱-串联质谱检测方法。以氘代试剂为内标，样品经酸化乙腈萃取后，平行定量浓缩仪浓缩，用正己烷脱脂，采用超高效液相色谱-串联质谱选择反应监测正离子模式测定，可同时对鱼肉中奎诺酮进行定性和定量。奎诺酮检出限可达0.5μg/kg，定量低限可达1.0μg/kg。在2.5～200ng/mL范围内峰强度与质量浓度的线性关系良好(r＞0.99)。方法的平均回收率在81.4%～111.1%之间。方法简单、快速、稳定、准确、灵敏度高，适合于实验室大批量检测水产品样品。

Rapid Simultaneous Determination of Eight Quinolone Residues in Eel by Syncore Analyst-Ultra Performance Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry

A method for the simultaneous determination of eight quinolone residues in eel was developed by syncore analyst-ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry(SA-UPLC-MS/MS).Deuterium substituted reagents were added as internal standards before sample extraction with acidified acetonitrile,followed by concentration using a Syncore Analyst and clean-up with hexane.Eight quinolone residues were analyzed by UPLC-MS/MS under positive ion mode.The limit of detection of the method for eight quinolones was 0.5μg/kg,and the limit of quantification was 1.0μg/kg.The calibration curves showed good linearity over the concentration range of 2.5-200 ng/mL(r > 0.99).The average recovery rates were 81.4%-11.1% across three spike levels(n = 6).This method was simple,rapid,stable,reliable,sensitive and suitable for the determination of bulk aquatic products.