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Enhancement of biohythane production from solid waste by co-digestion with palm oil mill effluent in two-stage thermophilic fermentation
Affiliation:1. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Songkhla 90112, Thailand;2. Department of Science, Faculty of Science and Technology, Prince of Songkla University, Pattani, 94000, Thailand;3. Department of Biology, Faculty of Science, Thaksin University, Phatthalung, 93210 Thailand;1. Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, National University of Malaysia (Universiti Kebangsaan Malaysia), 43600 Bangi, Selangor, Malaysia;2. Metabolic Engineering and Molecular Biology Research Lab iKohza, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia International Campus, Jalan Sultan Yahya Petra, 54100 Kuala Lumpur, Malaysia;3. Department of Microbiology, Masjed Soleyman Branch, Islamic Azad University, Masjed Soleyman, Iran;4. School of Biosciences and Biotechnology, Faculty of Science and Technology, National University of Malaysia (Universiti Kebangsaan Malaysia), 43600 Bangi, Selangor, Malaysia;1. Department of Environmental Health Sciences, Faculty of Public Health, Mahidol University, Bangkok 10400, Thailand;2. Center of Excellence on Environmental Health and Toxicology, Bangkok, Thailand;1. Biotechnology Program, Faculty of Science, Thaksin University, Phatthalung, 93210 Thailand;2. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Songkhla, 90112, Thailand;3. Department of Biology, Faculty of Science, Thaksin University, Phatthalung, 93210, Thailand;4. Research Center in Energy and Environment, Thaksin University, Phatthalung, 93210, Thailand;1. Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand;2. Research Group for Development of Microbial Hydrogen Production Process from Biomass, Khon Kaen University, Khon Kaen 40002, Thailand;3. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai 90112, Thailand;1. Department of Civil Engineering, University of Malaya, 50603, Kuala Lumpur, Malaysia;2. Department of Applied Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran;3. Department of Mechanical Engineering, University of Malaya, 50603, Kuala Lumpur, Malaysia;1. Biotechnology Program, Department of Biology, Faculty of Science, Thaksin University, Phatthalung 93210, Thailand;2. Agriculture Program, Faculty of Sciences and Technology, Nakhon Si Thammarat Rajabhat University, Nakhon Si Thammarat, 80280, Thailand;3. Research Center in Energy and Environment, Faculty of Science, Thaksin University, Phatthalung 93210, Thailand;4. Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, 40002, Thailand
Abstract:Improvement of biohythane production from oil palm industry solid waste residues by co-digestion with palm oil mill effluent (POME) in two-stage thermophilic fermentation was investigated. A two-stage co-digestion of solid waste with POME has biohythane production of 26.5–34 m3/ton waste. The co-digestion of solid waste with POME increased biohythane production of 67–114% compared to digestion POME alone. Co-digestion of solid waste with POME enhanced hydrolysis constant (kh) from 0.07 to 0.113 to 0.120–0.223 d−1. The hydrolysis constant (kh) of co-digestion was 10 times higher than the single digestion of solid waste. Clostridium sp. was predominated in the hydrogen stage, while Methanosphaera sp. was predominant in methane stage. The co-digestion of solid waste with readily biodegradable organic matter (POME) could significantly increase biohythane production with achieving the significant cost reduction for pretreatment of solid wastes.
Keywords:Thermophilic fermentation  Palm oil mill effluent  Co-digestion  Biohythane production  Solid waste residues  Microbial community
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