异硫氰酸丙烯酯毒杀松材线虫作用机制初步研究

对异硫氰酸丙烯酯毒杀松材线虫作用机制进行了初步研究。测定了其对线虫虫体形态、运动行为、体内总糖和蛋白质含量的影响,以及处理前后线虫体内过氧化氢酶(CAT)、乙酰胆碱酯酶(AChE)和谷胱甘肽-S-转移酶(GST)活性变化。结果表明:经异硫氰酸丙烯酯处理后的松材线虫虫体形态严重扭曲,运动形态出现由正常的S形变化为多节弯曲和螺旋状卷曲等异常状况,线虫行动随时间的推移变得迟缓,最终呈C形、新月形或直接死亡。随着异硫氰酸丙烯酯处理质量浓度的增大,线虫体内总糖和蛋白质含量显著上升,糖和蛋白质的代谢发生紊乱;线虫体内CAT、AChE和GST活性显著降低,且均低于对照组,表明线虫体内氧化和抗氧化作用失衡;细胞受到极大的影响,神经系统遭到破坏,解毒能力明显降低。研究结果可为研究利用异硫氰酸丙烯酯作为植物源杀线虫剂提供依据。

Preliminary study on the action mechanism of ally isothiocyanate against Bursaphelenchus xylophilus

A preliminary study on the mechanism of action of propylene isothiocyanate against Bursaphelenchus xylophilus was carried out. Its effects on the morphological changes and movement, the metabolism of total sugar and protein and activities of protective enzymes, catalase (CAT), acetylcholinesterase(AChE) and glutathione thiotransferase(GST) enzyme from B. xylophilus were evaluated. Ally isothiocyanate interfered with body morphology of B. xylophilus, which was seriously distorted by AITC, and its movement morphology changed from normal S shape to multi-section bending and spiral curl. Its locomotion capacity became slow with time, and finally showed C shape, crescent shape or dead form. With the increase of the concentration of isopropyl isothiocyanate, the total sugar and protein contents of nematodes increased significantly, and the metabolism of sugar and protein was disordered. The activities of CAT, AChE and GST in nematodes were significantly lower than those in the control groups. It indicated that the oxidative and antioxidative effects of nematodes were imbalanced. The cells were significantly affected, and the nervous system was destroyed. The detoxification ability was also dramatically reduced. Those results can be used to evaluate the use of propylene isothiocyanate as a plant-derived nematicide.