家种及野生秦艽、麻花秦艽的rRNA基因间隔区PCR产物电泳图谱的初步研究

目的：分析甘肃不同地区家种及野生中药材秦艽Gentiana macrophylla、麻花秦艽G.straminea中提取的DNA中rRNA基因内转录间隔区PCR扩增产物的电泳图谱，为秦艽、麻花秦艽等不同品种鉴别和品质评价从分子水平提供依据。方法：提取中药材家种及野生秦艽、麻花秦艽的核基因组DNA，利用合成的特异性PC及引物对所提取的DNA中rRNA基因内转录间隔序列进行nPCR扩增，扩增产物行琼脂糖凝胶电泳以得到电泳图谱并进行分析。结果：琼脂糖凝胶电泳图谱显示不同秦艽DNA中rRNA基因内转录间隔区长度均在360bp左右，已具备足够的遗传信息量进行碱基序列分析。结论：不同秦艽DNA中rRNA基因内转录间隔区PCR扩增产物可作为从分子水平进行鉴别的标记之一。

Primary Study on the Electrophoresis Atlas of the PCR Products of the Internal Transcribed Spacerl Regions of the rRNA Gene from Home-planted and Wild Gentiana macrophylla, G. straminea

Objective: To analyse the electrophoresis atlas of the PCR amplified products of the internal transcribed spacerl regions of the rRNA gene in DNA extracted from home-planted and wild Gentiana macrophylla, G. straminea in Gansu. Methods; With synthetic peculiar PCR primer, internal transcribed spacerl sequences of rRNA gene were amplified in DNA extracted from home-planted and wild Gentiana macrophylla, G. straminea. And then the electrophoretic atlas of nPCR amplified products on the agar sugar gel were analysed. Result; The electrophoresis atlas showed that the lengths of the internal transcribed spacerl regions of the rRNA gene in DNA from Gentiana macrophylla, G. straminea had the same region 360bp. The length had enough genetic information to analyse base sequence. Conclusion; The PCR amplified products electrophoretic atlas of internal transcribed spacerl regions of the rRNA gene in DNA from Gentiana macrophylla, G. straminea can be used as one of signs for identification on molecule level.