Mapping of two new brown planthopper resistance genes from wild rice |
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Authors: | Wang Buna Huang Zhen Shu Lihui Ren Xiang Li Xianghua He Guangcun |
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Affiliation: | (1) Key Laboratory for Plant Developmental Biology, College of Life Science, Wuhan University, 430072 Wuhan, China;(2) National Key Laboratory of Genetic Improvement of Crop, Huazhong Agricultural University, 430064 Wuhan, China |
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Abstract: | A brown planthopper (BPH) resistance line, B5, derived its resistance genes from the wild riceOryza officinalis Wall exwatt, was hybridized with Taichung Native 1, a cultivar highly susceptible to BPH. A mapping population composed of
randomly selected 167 F2 individuals was used for determining the BPH resistance genes by the restriction fragment length polymorphism analysis (RFLP).
Bulked segregant analysis was conducted to identify RFLP makers linked to the BPH resistance genes in B5. The results indicated
that the markers linked to BPH resistance are located at two genomic regions on the long arm of chromosome 3 and the short
arm of chromosome 4, respectively. The existence of the two loci was further assessed by the quantitative trait locus (QTL)
analysis. We located the two loci at a 3.2 cM interval between G1318 and R1925 on chromosome 3 and a 1.2 cM interval between
C820 and S11182 on chromosome 4. Comparison with the BPH genes that have been reported indicated that the BPH resistance genes
in B5 are novel. These two genes may be useful BPH resistance resource for rice breeding. Furthermore, the mapping of the
two genes is useful for cloning the BPH resistance genes. |
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Keywords: | Oryza sativa L brown planthopper resistance gene molecular marker gene localization |
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