| 正交试验优选血三七总黄酮的提取工艺研究 |
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| 引用本文： | 王金翠,胡雄彬,唐甜甜,韩 萌,刘新义.正交试验优选血三七总黄酮的提取工艺研究[J].现代药物与临床,2014,29(4):370-372. |
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| 作者姓名： | 王金翠 胡雄彬 唐甜甜 韩 萌 刘新义 |
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| 作者单位： | 桑植县民族中医院 药剂科，湖南 桑植 427100;中南大学湘雅二医院 临床药学研究所，湖南 长沙 410011;中南大学湘雅二医院 临床药学研究所，湖南 长沙 410011;中南大学湘雅二医院 临床药学研究所，湖南 长沙 410011;中南大学湘雅二医院 临床药学研究所，湖南 长沙 410011 |
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| 基金项目： | 湖南省中医药管理局课题（2013139） |
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| 摘 要： | 目的优选血三七Polygonum amplexicaule var.sinense中总黄酮的提取工艺。方法以乙醇体积分数、提取时间、乙醇倍数、提取次数为因素,以总黄酮提取率和浸膏得率为指标,采用正交试验优选提取工艺。结果最佳条件为用10倍70%乙醇,提取3次,每次1.5 h。结论优选的工艺稳定、合理,可用于血三七中总黄酮的提取。
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| 关 键 词： | 血三七 总黄酮 浸膏得率 正交试验 |
Extraction optimization of total flavonoids from Polygonum amplexicaule var. sinense by orthogonal test |
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| WAMG Jin-cui,HU Xiong-bin,TANG Tian-tian,HAN Meng and LIU Xin-yi.Extraction optimization of total flavonoids from Polygonum amplexicaule var. sinense by orthogonal test[J].Drugs & Clinic,2014,29(4):370-372. |
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| Authors: | WAMG Jin-cui HU Xiong-bin TANG Tian-tian HAN Meng and LIU Xin-yi |
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| Affiliation: | Department of Pharmacy, Sangzhi County National Hospital of Chinese Medicine, Sangzhi 427100, China;Institution of Clinical Pharmacy, The second Xiangya Hospital of Central South University, Changsha 410011, China;Institution of Clinical Pharmacy, The second Xiangya Hospital of Central South University, Changsha 410011, China;Institution of Clinical Pharmacy, The second Xiangya Hospital of Central South University, Changsha 410011, China;Institution of Clinical Pharmacy, The second Xiangya Hospital of Central South University, Changsha 410011, China |
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| Abstract: | Objective To optimize the extraction technology of total flavonoids from Polygonum amplexicaule var. sinense. Methods The extraction technology of total flavonoids was optimized by orthogonal test using ethanol concentration, ethanol ratio, extracting time, and extracting times as factors with the extraction of total flavone and extract yield as indexes. Results The optimal extraction technology was as follows: 10-folds of 70% ethanol, extracting for three times, and 1.5 h each time. Conclusions The optimal technology is stable and feasible for the extraction of total flavonoids from P. amplexicaule var. sinense. |
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| Keywords: | Polygonum amplexicaule D Don var sinense Forb et Hemsl ex Stew total flavonoids extract yield orthogonal test |
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