| 脑微血管内皮细胞体外缺血再灌注模型的建立及牛胆酸的保护作用 |
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| 引用本文： | 高永红,袁拯忠,牛福玲,崔巍,朱陵群,王硕仁,.脑微血管内皮细胞体外缺血再灌注模型的建立及牛胆酸的保护作用[J].中国中药杂志,2005,30(12):930-932. |
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| 作者姓名： | 高永红 袁拯忠 牛福玲 崔巍 朱陵群 王硕仁 |
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| 作者单位： | 1. 北京中医药大学,东直门医院,脑病实验室,北京,100700
2. 北京中医药大学,中医内科学教育部重点实验室,北京,100700 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 目的:建立脑微血管内皮细胞体外缺血再灌注模型,并观察了牛胆酸对其影响。方法:体外培养大鼠脑微血管内皮细胞,以氧糖剥夺(Krebs液)再复氧复糖模型模拟缺血再灌注损伤,并用牛胆酸进行了干预,用MTT比色法测定A值代表细胞生存活性。结果:氧糖剥夺4h再复氧复糖12h可造成培养大鼠脑微血管内皮细胞损伤,牛胆酸干预后A值明显高于模型组(P<0.01)。结论:牛胆酸可显著减轻脑微血管内皮细胞体外模拟缺血再灌注细胞损伤。
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| 关 键 词： | 脑微血管内皮细胞 大鼠 缺血再灌注 牛胆酸 |
| 文章编号： | 1001-5302(2005)12-0930-03 |
| 收稿时间： | 2004/12/6 0:00:00 |
The protective effect of cholic acid on an in vitro injury model of ischemia-reperfusion in cerebral microvascular endothelial cells |
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| GAO Yong-hong;YUAN Zheng-zhong;NIU Fu-ling;GUI Wei;ZHU Ling-qun;WANG Shuo-ren.The protective effect of cholic acid on an in vitro injury model of ischemia-reperfusion in cerebral microvascular endothelial cells[J].China Journal of Chinese Materia Medica,2005,30(12):930-932. |
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| Authors: | GAO Yong-hong;YUAN Zheng-zhong;NIU Fu-ling;GUI Wei;ZHU Ling-qun;WANG Shuo-ren |
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| Affiliation: | The cerebropathia laboratory of Dongzhinen Hospital, Beijing University of Traditional Chinese Medicine, Beijing 100700, China. |
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| Abstract: | Objective: To establish an in vitro injury model of ischemia-reperfusion in cerebral microvascular endothelial cells of rats and observe the protective effect of cholic acid. Method: Cultured rat microvascular endothelial cells were subjected to the oxygen-glucose deprivation (OGD) (Krebs solution) and recovery of oxygen-glucose, which simulated in vitro ischemia and reperfusion injury,and treated with cholic acid. The A value was measured with MTT chromatometry. Result: Cultured cells were impaired after OGD for 4 hours and recovery of oxygen-glucose for 12 hours, the A value of the cells treated with cholic acid was significantly higher than that of the cells without treatment(P<0.01). Conclusion: Cholic acid could obviously protect rat cerebral microvascular endothelial cells from injury induced by an in vitro ischemia-reperfusion. |
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| Keywords: | Cerebral microvascular endothelial cell rat ischemia-reperfusion cholic acid |
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