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| 阳离子交换色谱柱上聚合血红蛋白研究 | |
| 引用本文： | 王翔,黄磊,杨成民,王劲峰.阳离子交换色谱柱上聚合血红蛋白研究[J].生物医学工程学杂志,2008,25(5). |
| 作者姓名： | 王翔 黄磊 杨成民 王劲峰 |
| 作者单位： | 1. 天津大学,药学院,天津,300071;天津工业大学,材料化工学院,天津,300160 2. 天津工业大学,材料化工学院,天津,300160 3. 天津协和生物技术发展研究所,天津,300457 4. 天津大学,药学院,天津,300071 |
| 摘 要： | 采用柱上聚合的方法制备血红蛋白溶液，解决传统血红蛋白聚合过程中由于戊二醛的活性过高导致平均分子量大、产物分子量分布宽的问题。该方法利用阳离子交换剂对修饰度（聚合度）小的血红蛋白吸附能力大的原理，使其在柱上富积，同时加入戊二醛进行聚合反应。结果表明该方法能比较有效地缩小聚合血红蛋白的分子量分布。 |
| 关 键 词： | 聚合血红蛋白 柱上聚合反应 阳离子交换色谱 分子量分布 |
Study of Polymerizing Hemoglobin on Cation Exchange Chromatography |
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| Wang Xiang,Huang Lei,Yang Chenming,Wang Jinfeng.Study of Polymerizing Hemoglobin on Cation Exchange Chromatography[J].Journal of Biomedical Engineering,2008,25(5). | |
| Authors: | Wang Xiang Huang Lei Yang Chenming Wang Jinfeng |
| Affiliation: | College of Pharmaceuticals and Biotechnology;Tianjin University;Tianjin 300071;China;Institute of Tianjin Union Biotechnology Development Company;Tianjin 300457;China;College of Material Science and Chemical Engineering;Tianjin Polytechnology University;Tianjin 300160;China |
| Abstract: | Poly-hemoglobin is the active component of hemoglobin-based blood substitutes. The excess reactivity of glutaraldehyde with hemoglobin in solution leads to poly-hemoglobin of a wide molecular weight distribution and a high average molecular weight. A new polymerization method has been tested to decrease the molecular weight distribution and the average molecular weight. The poly-hemoglobin with lower degree of modification (polymerization) was found enriched on the cation exchange columns and further polyme... |
| Keywords: | Poly-hemoglobin Polymerization Cation exchange chromatography Molecular weight distribution |
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