| qPCR-HRM曲线分析技术与普通PCR加直接测序法检测胶质瘤EGFR突变比较 |
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| 引用本文： | 熊亮,梁朝峰,陈川,罗伦,凌聪,拳峰,蔡梅钦,史志东.qPCR-HRM曲线分析技术与普通PCR加直接测序法检测胶质瘤EGFR突变比较[J].新医学,2012,43(5):327-330. |
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| 作者姓名： | 熊亮 梁朝峰 陈川 罗伦 凌聪 拳峰 蔡梅钦 史志东 |
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| 作者单位： | 中山大学附属第三医院神经外科,510630 |
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| 摘 要： | 目的:比较实时聚合酶链反应-高分辨率融解(qPCR-HRM)曲线分析技术和普通PCR法加直接测序法检测胶质瘤患者EGFR基因突变类型,探讨适用于临床的EGFR基因突变检测方法,为胶质瘤患者术后放射、化学治疗及预后判断研究提供可靠的依据。方法:用普通PCR加测序法与qPCR-HRM曲线分析技术检测胶质瘤患者EGFR基因突变类型,检测结果比较采用卡方检验。结果:两种方法检测EGFR外显子19基因突变结果比较差异无统计学意义,且qPCR-HRM曲线分析技术比直接测序法更快捷、灵敏。结论:与直接测序法相比,qPCR-HRM曲线分析技术可能更适用于临床检测胶质瘤患者标本EGFR突变性质。
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| 关 键 词： | 表皮生长因子受体 基因突变 普通PCR DNA测序法 实时聚合酶链反应-高分辨率融解曲线分析技术 |
Application of qPCR-HRM curve analysis and common-PCR with DNA sequencing in detection of EGFR mutations in glioma tissues |
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| XIONG Liang
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LIANG Chao-feng
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CHEN Chuan
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LUO Lun
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LING Cong
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QUAN Feng
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CAI Mei-qin
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SHI Zhi-dong.Application of qPCR-HRM curve analysis and common-PCR with DNA sequencing in detection of EGFR mutations in glioma tissues[J].New Chinese Medicine,2012,43(5):327-330. |
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| Authors: | XIONG Liang LIANG Chao-feng CHEN Chuan LUO Lun LING Cong QUAN Feng CAI Mei-qin SHI Zhi-dong |
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| Affiliation: | .( Department of Neurosurgery, The Third Affiliated Hospital of SUN Yat-sen University, Guangzhou 510630, China ) |
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| Abstract: | Objective： To compare the qPCR-HRM and common-PCR with DNA sequencing in detection of EGFR mutations. Methods： EGFR mutation were detected by qPCR-HRM and common-PCR with DNA sequen- cing in glioma. Chi-square test was used to analyze the consistency. Results： There was no significant difference between the two methods to detected Exon 19 mutation in EGFR. However, qPCR-HRM was more convenient and sensitive than DNA sequencing. Conclusion： qPCR-HRM is suitable in detection of EGFR mutations in glioma. |
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| Keywords: | Epidermal growth cing Quantitative factor receptor Mutations Common polymerase chain reaction DNA Sequenpolymerase chain reaction-high resolution melting curve analysis |
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