| 芹菜素抑制蛋白激酶B活性诱导人卵巢癌细胞凋亡的机制研究 |
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| 引用本文： | 任秀聪,李彦敏.芹菜素抑制蛋白激酶B活性诱导人卵巢癌细胞凋亡的机制研究[J].现代中西医结合杂志,2010,19(14):1709-1712. |
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| 作者姓名： | 任秀聪 李彦敏 |
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| 作者单位： | 河北省石家庄市第一医院,河北,石家庄,050011 |
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| 基金项目： | 河北省石家庄市科技攻关课题 |
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| 摘 要： | 目的探讨芹菜素通过抑制蛋白激酶B活性诱导人卵巢癌细胞凋亡的作用机制。方法采用细胞形态学观察、DNA ladder、聚丙烯酰胺凝胶电泳检测Caspasse-3,Akt,磷酸化Akt,Bad及磷酸化Bad蛋白表达并测定Akt激酶活性。结果芹菜素明显抑制卵巢癌细胞的生长;DNA琼脂糖凝胶电泳检测到典型的DNA梯形条带;Western blotting方法检测到Caspase-3的活性片段,并呈明显的时间依赖关系;芹菜素可以时间依赖方式抑制与细胞增殖相关的Akt及Akt下游促凋亡蛋白Bad的磷酸化,但对总Akt及总Bad蛋白的表达无影响。结论芹菜素通过抑制Akt活性能诱导人卵巢癌细胞发生凋亡。
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| 关 键 词： | 芹菜素 Akt 凋亡 卵巢癌 |
Mechanism study of apigenin inducing apoptosis of human ovarian cancer cells through inhibiting protein kinase B activity |
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| Ren Xiucong,Li Yanmin.Mechanism study of apigenin inducing apoptosis of human ovarian cancer cells through inhibiting protein kinase B activity[J].Modern Journal of Integrated Chinese Traditional and Western Medicine,2010,19(14):1709-1712. |
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| Authors: | Ren Xiucong Li Yanmin |
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| Affiliation: | Ren Xiucong,Li Yanmin(The First Hospital of Shijiazhuang City,Shijiazhuang 050011,Hebei,China) |
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| Abstract: | Objective It is to approach the mechanism of apigenin inducing apoptosis of human ovarian cancer cells through inhibiting protein kinase B activity.Methods Morphology and DNA ladder were observed.The protein expressions of Caspase-3,Akt,phosphorylated Akt,Bad and phosphorylated Bad were detected with polyacrylamide gel electrophoresis and the activity of Akt kinase was determined.Results Apigenin could obviously inhibit the growth of ovarian cancer cells.Typical ladder-shaped strap was detected with agarose... |
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| Keywords: | Akt |
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