转铁蛋白受体在肺癌中的表达

目的探讨转铁蛋白受体（transferrin receptor，TfR／CD71）在肺癌细胞株和肺癌组织中的表达及其与病理类型的关系。方法选取手术切除肺癌标本5l例和正常肺组织10例，免疫组织化学Envision法检测TfR／CD71的表达，分析TfR／CD71的表达与肺癌病理类型的相关性。同时细胞化学染色检测人肺腺癌细胞系A549细胞爬片TfR／CD71的表达。结果51例肺癌组织中TfR／CD71阳性表达26例50．98％（26／51）]，其中腺癌18例69．23％（18／26）]，鳞癌8例53．33％（8／15）]；10例正常肺组织TfR／CD71表达均为阴性；肺癌组织和正常肺组织TfR／CD71表达差异有统计学意义（P〈0．01）；TfR／CD71表达与肺癌组织学类型呈正相关（r=0．497，P〈0．01），腺癌阳性率显著高于其他组织学类型（P〈0．01）。结论TfR／CD71在相对正常肺组织呈灶性弱表达或阴性，在肺癌细胞爬片和肺癌组织中均呈高表达，且主要是表达于肺癌细胞的胞膜，胞膜邻近的胞浆也有表达。TfR／CD71的表达与肺癌的组织学类型有关，腺癌表达明显高于其他组织学类型。

The expression of transferrin receptor in lung cancer

Objective To investigate the expression of TfR （CD71） and its correlation with pathological type of lung cancer. Methods Fifty one cases of lung cancer and 10 cases of normal lung tissues were selected. Immunohistochemisty Envision method was used to assess the expression of TfR （ CD71 ）. Statistical analysis was used to evaluate the relationship between TfR （CD71） expression and pathological type of lung cancer. Meanwhile, immunocytochemistry was used to detect TfR （CD71） expression on lung cancer cell line A549. Results The positive rate of TfR （ CD71 ） was 50. 98 % （ 26/51 ） in 51 cases of lung cancers. Among them, the positive rate of TfR （ CD71 ） expression was 69. 23% （18/26） in adenocarcinoma, and 53.33% （8/15） in squamous cell carcinoma, respectively. Ten eases of normal lung tissues were negative for TfR （ CD71 ） expression. Statistical analysis of TfR （ CD71 ） expression in lung cancer tissues and normal lung tissues showed a significant difference （ P 〈 0. 01 ）. TfR （ CD71 ） expression was correlated with histological types of lung cancer （ r =0. 497, P 〈0. 01 ）. Positive rate in adenocarcinoma was significantly higher than other histological types （ P 〈 0. 01 ）. Conclusions The expression of TfR （CD71） was detected in lung cancer cells, but not detected in normal lung tissue. TfR （CD71） was mainly expressed in lung cancer cell membrane and cytoplasm. The positive expression of TfR （ CD71 ） was related to the pathological type of lung cancer, and adenocarcinoma was significantly higher than other types.