塞络通胶囊对多发梗死性痴呆大鼠认知功能障碍的干预作用

目的:研究塞络通胶囊(塞络通)对多发梗死性痴呆(multi-infarct dementia,MID)大鼠学习记忆功能的改善作用及其机制.方法:SD大鼠随机分为假手术组、模型组、阳性药组以及塞络通高、低剂量组.采用SD大鼠颈内动脉注射微球血管栓塞剂的方法建立多发梗死性痴呆模型;手术成功后第10天,大鼠分别灌胃给予蒸馏水、盐酸美金刚(20 mg· kg-1)以及高、低剂量的塞络通(16.5,33.0 mg·kg-1),每日1次,连续60 d;检测塞络通不同剂量对模型大鼠Morris水迷宫潜伏期、路径长度,以及脑组织胆碱乙酰转移酶(ChAT)和乙酰胆碱酯酶(AchE)活性的影响.结果:与假手术组比较,多发梗死性痴呆大鼠在Morris水迷宫实验中的潜伏期及路径长度均明显延长(P＜0.05或P＜0.01),脑组织ChAT活性明显降低(P＜0.05);塞络通干预60 d后明显缩短模型大鼠在Morris水迷宫实验中的潜伏期及路径长度(P＜0.05或P＜0.01);并且明显降低脑组织AchE活性面升高ChAT活性(P＜0.05或P＜0.01).结论:塞络通对多发梗死性痴呆大鼠认知功能障碍具有一定程度的改善作用,作用机制可能与其对脑组织ChAT活性以及AchE活性不同的调节相关.

Effect of Sailuotong capsule on intervening cognitive dysfunction of multi-infarct dementia in rats

Objective: To study the effect of Sailuotong capsule (Sailuotong) on learning and memory functions of multi-infarct dementia (MID) rats and its mechanism. Method: All SD rats were divided into five groups, namely the sham operation group, the model group, the positive group, the low dosage Sailuotong-treated group and the high dosage Sailuotong-treated group. The multi-infarct dementia model was established by injecting the micro-sphere vascular occlusive agent. On the 10^th day after the successful operation, the rats were administered intragastrically with distilled water, memantine hydrochloride (20 mg·kg^-1) and Sailuotong (16.5 mg·kg^-1 and 33.0 mg·kg^-1) once a day for 60 days respectively, in order to detect the effect of Sailuotong in different doses on the latent period and route length in Morris water maze and the activities of choline acetyltransferase (ChAT) and acetylcholinesterase (AchE) in brain tissues. Result: Compared with the sham operation rats, it had been observed that the latent period and route length of MID rats in Morris water maze were significantly increased (P<0.05 or P<0.01), and the activity of ChAT in brain tissues was significantly decreased (P<0.05). After the intervention with Sailuotong for sixty days, the latent period and route length of MID rats in Morris water maze significantly shrank (P<0.05 or P<0.01). Additionally, Sailuotong decreased AchE activity, while increasing ChAT activity in brain tissues of MID rats (P<0.05 or P<0.01). Conclusion: Sailuotong capsule can improve cognitive dysfunction of MID rats to some extent. Its mechanism may be related to its different regulation of activities of ChAT and AchE in brain tissues.