市售鸡肉中沙门菌分离株多重耐药谱测定

为了解我国市售鸡肉中沙门菌多重耐药状况及耐药程度，对国家食源性疾病监测网从市售鸡肉中分离到的51株沙门菌进行耐药检测，并对多重耐药谱进行分析。利用NccLs(National Committee of Clinical Labaratory Standard)推荐的纸片法进行耐药检测，51株分离菌株全部对至少3种以上的抗生素耐药，属多重耐药株，耐5种抗生素的9株(17.6％)，耐6～9种抗生素的12株(23．5％)，耐10种以上抗生素的9株(17．6％)。51株沙门菌对万古霉素、红霉素、苯唑西林都具有耐药性(100％)，对其它抗生素的耐药情况是：萘啶酮酸(52.9％)、磺胺(35．3％)、链霉素(33．3％)、四环素(29.4％)、氨苄青霉素(23．5％)、羧苄西林(21.6％)、阿莫西林(19．6％)、吡拉西林(19．6％)、美唑西林(17．6％)、强力霉素(17．6％)、氯霉素(13．7)和头孢噻吩(9．8％)。有3株菌对庆大霉素耐药，有2株菌对丁胺卡那霉素和甲氧苄胺嘧啶耐药，有1株菌对复方新诺明耐药。分离出1株耐15种抗生素的鼠伤寒沙门菌，其耐药谱与超级耐药鼠伤寒沙门菌DTl04的耐药谱类似。从8省市市售鸡肉中分离的51株沙门菌均为多重耐药株，提示我国畜牧养殖业在使用抗生素方面存在严重问题，应加强对饲料添加剂使用的安全性管理，合理使用抗生素。

Characterization of multiple-antimicrobial-resistant Salmonella isolated from retail chicken in China

To determine the antibiotic susceptibility of Salmonella isolates from retail chicken and evaluate the spectrum of their multiple antimicrobial resistance , fifty one strains of identified Salmonella isolates were analyzed for minimal inhibitory concentrations (MICs) with 30 kinds of antibiotics using the disk diffusion method All of the strains were resistant to at least three antibiotics. Eight strains (15 7%) were resistant to three kinds, thirteen strains (25 5%) to four, nine strains (17 6%) to five, twelve strains (23 5%) to six to nine and nine strains (17 6%) to ten antimicrobial agents All of the 51 strains were resistent to vancomycin,erythromycin and mezlocillin. Resistance to the following antibiotics was common among isolates: nalidixic acid (52 9%), sulfonamides(35 9%), streptomycin (33 3%), tetracycline (29 4%), ampicillin (23 5%), carbenicillin (21 6%), amoxicillin (19 6%), piperacillin (19 6%), mezlocillin (17 6%), doxycycline (17 6%) and chloramphenicol (13 7%). It is important that one isolate of Salmonella typhimurium isolated from the retail chicken in Fujian province was resistant to fifteen antimicrobial agents, which exhibiting the antimicrobial resistance profile similar to that of Salmonella typhimurium DT104. These data indicate that the fifty one Salmonella isolates recovered from retail raw chicken are commonly resistant to multiple antibiotics.