| Affiliation: | 1. Curso de Pós–Graduação em Ciências Biológicas--Bioquímica, Instituto de Ciências Básicas de Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600 (anexo), Porto Alegre, RS, CEP: 90035--003, Brazil;2. Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, Instituto de Ciências Básicas de Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600 (anexo), Porto Alegre, RS, CEP: 90035--003, Brazil;3. South–American Office for Anticancer Drug Development (SOAD), Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos, 2350, Porto Alegre, RS, CEP: 90035--007, Brazil;4. Centro Universitário Feevale, RS 239, 2755, Novo Hamburgo, RS, CEP: 93352--000, Brazil |
| Abstract: | The ethanolic extract of Symphyopappus casarettoi syn. Eupatorium casarettoi was partionated in hexanic, chloroformic and methanolic fractions. Extract and fractions were tested for their antioxidant activity in vitro and ex vivo assays. The methanolic fraction showed a higher antioxidant potential compared to the others fractions, which was correlated with its total phenol content. In addition, the ethanolic extract and the methanolic fraction attenuated ex vivo iron-induced cell death, quantified by lactate dehydrogenase leakage, and effectively protected against lipid damage induced by iron. These findings suggest that the ethanolic extract of S. casarettoi inflorescence and its methanolic fraction have in vitro and ex vivo antioxidant properties. |
