脂多糖对大鼠肺微血管内皮细胞[Ca2+]i和Gq蛋白的影响及山莨菪碱的干预

目的:观察脂多糖对大鼠肺微血管内皮细胞(RPMVECs)Ca²⁺]_i和Gq蛋白的影响及山莨菪碱的干预作用。方法:分离、培养并鉴定Wistar大鼠RPMVECs;应用Fura-2/AM法测定RPMVECsCa²⁺]_i;流式细胞仪技术测定RPMVECsGq蛋白。结果:①LPS作用于RPMVECs30min和90min后,Ca²⁺]_i显著高于对照组;Gq蛋白显著低于对照组。②山莨菪碱可抑制LPS的上述作用。结论:①LPS致RPMVECsCa²⁺]_i增加和Gq蛋白下降;②山莨菪碱通过抑制LPS诱导RPMVECsCa²⁺]_i增加和Gq蛋白下降的作用而保护其内皮屏障功能。

2+ and Gq protein in pulmonary microvascular endothelial cells of rats and the interferring action of anisodamine

AIM:To study the effect of lipopolysaccharide(LPS) on cytosolic Ca²⁺ and Gq protein in pulmonary microvascular endothelial cells of rats (RPMVECS) and the interferring action of anisodamine.METHODS:RPMVECS of Wistar rats were isolated, cultured and identified. The concentration of cytosolic Ca²⁺ and Gq protein were measured by Fura-2/AM and flow cytometry, respectively.RESULTS:(1) Compared with the control group, the level of cytosolic Ca²⁺ increased and the level of Gq protein decreased significantly at 30 min and 90 min after stimulation of LPS;(2) Increase in cytosolic Ca²⁺ and decrease in Gq protein in RPMVECS were inhibited by anisodamine. CONCLUSION:LPS induced the increase in cytosolic Ca²⁺ and the decrease in Gq protein in RPMVEC, which was inhibited by anisodamine.