| 铜离子对海参精氨酸激酶活力与结构的影响 |
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| 引用本文： | 刘陶陶,王希成.铜离子对海参精氨酸激酶活力与结构的影响[J].海洋科学,2011,35(1):17-21. |
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| 作者姓名： | 刘陶陶 王希成 |
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| 作者单位： | 1. 清华大学,生命科学院,北京,100084
2. 清华大学,生命科学院,北京,100084;清华大学生命科学院,蛋白质科学教育部重点实验室,北京,100084 |
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| 摘 要： | 精氨酸激酶(Arginine kinase,EC2.7.3.3)是无脊椎动物能量代谢所必需的重要的酶.海参精氨酸激酶是一种特殊的双亚基精氨酸激酶.本文研究了在二价铜离子作用下,海参精氨酸激酶催化活性与结构的变化.结果表明,一定浓度的铜离子,可以抑制精氨酸激酶的活力,并引起酶二级结构与三级结构的变化,引起疏水面暴露,并导...
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| 关 键 词： | 精氨酸激酶(Arginine kinase EC2.7.3.3) Cu2+ 聚沉 去折叠 |
| 收稿时间： | 2010/6/25 0:00:00 |
| 修稿时间： | 9/7/2010 12:00:00 AM |
Effects of Cu2+ on Arginine kinase: activity changes, conformational changes, and aggregation |
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| LIU Tao-tao,WANG Xi-cheng.Effects of Cu2+ on Arginine kinase: activity changes, conformational changes, and aggregation[J].Marine Sciences,2011,35(1):17-21. |
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| Authors: | LIU Tao-tao WANG Xi-cheng |
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| Affiliation: | LIU Tao-tao1,WANG Xi-cheng1,2(1.School of Life Sciences,Tsinghua University,Beijing 100084,China,2.Protein Science Laboratory of the Min-istry of Education,School of Life Sciences,China) |
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| Abstract: | Arginine kinase (AK) plays an important role in the cellular energy metabolism of invertebrate. AK from sea cucumber Stichopus japonicus is dimeric. The effects of Cu2+ on AK were studied by measuring activity changes, kinetic time course of inactivity, far-UV circular dichroism spectra, fluorescence spectra, and turbidity changes at 400nm. These results suggested that Cu2+ caused AK inactivation accompanied by conformational change, exposure of hydrophobic surface, and aggregation. The effects of Cu2+ on AK are distinctive compared with other metal divalent ions. |
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| Keywords: | Arginine kinase Cu2+ aggregation unfolding |
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