过量碘对原代培养的猪甲状腺细胞凋亡的诱导作用

目的观察过量碘对原代培养的猪甲状腺细胞凋亡的诱导作用,并对其机制作初步探讨。方法取健康的猪甲状腺细胞培养24 h后,加入不同浓度的碘化钾,观察细胞的形态改变;琼脂糖凝胶电泳和流式细胞术分析DNA的降解;采用MDA法测定细胞膜的脂质过氧化程度。结果加入碘化钾后6 h左右,可见细胞凋亡,48 h左右达到高峰;琼脂糖凝胶电泳出现典型的梯状带;流式细胞术可见亚二倍体高峰,细胞凋亡率随碘化钾浓度的升高而升高(P<0.05);脂质过氧化产物丙二醛含量随碘化钾浓度的升高而升高(P<0.05)。结论过量碘化钾诱导原代培养的猪甲状腺细胞凋亡,呈剂量依赖关系,可能通过活性氧机制启动凋亡。

Iodide excess induces apoptosis in primary cultured pig thyroid cells

Objective To study the apoptosis induced by excess iodide.Methods We employed the primary cultured pig thyroid cells.The apoptosis was evaluated by AQ-stained fluorescin,DNA ladder by gel electrophoresis and subdiploidy by propidium iodide-stained flow cytometry.The level of lipid peroxide under KI treatment was determined by measuring the production of thiobarbituric acid reactive substances.Results Thyroid cells treated with iodide excess of different concentration underwent apoptosis.Iodide displayed a dose-dependent apoptosis index and a dose-dependent generation of TBARS levels.Conclusions Iodide excess induces apoptosis in the primary cultured pig thyroid cells.This type of apoptosis seems to be activated by reactive oxygen species.