| 非衍生化-气相色谱法测定羊奶中短中链游离脂肪酸 |
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| 引用本文： | 晏慧莉,张富新,李延华,贾润芳,李龙柱,艾对,杨吉妮.非衍生化-气相色谱法测定羊奶中短中链游离脂肪酸[J].食品科学,2014,35(16):138-142. |
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| 作者姓名： | 晏慧莉 张富新 李延华 贾润芳 李龙柱 艾对 杨吉妮 |
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| 作者单位： | 1.陕西师范大学食品工程与营养科学学院，陕西 西安 710062；;
2.富平县畜牧技术推广中心，陕西 富平 711700 |
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| 基金项目： | “十二五”农村领域国家科技项目(2013BAD18B00);公益性行业(农业)科研专项(201103038);陕西省农业攻关项目(2014K01-17-05) |
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| 摘 要： | 建立羊奶中短中链游离脂肪酸（free fatty acids，FFA）的非衍生化-气相色谱分析方法。其测定过程包括
羊奶中脂肪的提取、FFA的分离和气相色谱测定3 个部分。首先用乙醇-乙醚-正庚烷将羊奶中脂质进行提取，然后
采用氨丙基固相萃取柱将FFA进行分离，最后用气相色谱法测定FFA含量。采用外标法测定羊奶中FFA的回收率，
以此作为FFA的校对系数。结果表明，羊奶中长链FFA的回收率较高，而短链FFA的回收率较低，尤其是C2︰0和C4︰0回
收率仅为23.9%和78.5%，但其回收率相对稳定，相对标准偏差小于0.04%，可校对测定过程中FFA的损失。
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| 关 键 词： | 羊奶 气相色谱 固相萃取 游离脂肪酸 |
Determination of Free Fatty Acids in Goat Milk by Gas Chromatography without Derivatization |
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| YAN Hui-li,ZHANG Fu-xin,LI Yan-hua,JIA Run-fang,LI Long-zhu,AI Dui,YANG Ji-ni.Determination of Free Fatty Acids in Goat Milk by Gas Chromatography without Derivatization[J].Food Science,2014,35(16):138-142. |
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| Authors: | YAN Hui-li ZHANG Fu-xin LI Yan-hua JIA Run-fang LI Long-zhu AI Dui YANG Ji-ni |
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| Affiliation: | 1. College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710062, China;
2. Husbandry Technology Promotion Center of Fuping County, Fuping 711700, China |
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| Abstract: | A method was developed for the determination of free fatty acids (FFAs) in goat milk by gas chromatography (GC)
without derivatization. The process includes three parts: the extraction of goat milk lipids, the separation of FFAs and the
determination by gas chromatography. Firstly, the milk lipids were extracted with ethanol/ether/n-heptane, and then FFAs
were isolated on an aminopropyl column. Finally, the FFAs were identified and determined by gas chromatography. The
external standard method was applied to determine the recoveries of FFAs in the goat milk, which acted as the calibration
coefficients of FFAs. The results showed that the recoveries of long chain FFAs were higher than those of short chain FFAs,
especially for C2︰0 and C4︰0 with values of 23.9% and 78.5%, respectively. The recoveries were stable with relative standard
deviations blew 0.04%. |
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| Keywords: | goat milk gas chromatography solid phase extraction free fatty acids |
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